Studies On Design And Expression Of Small Peptide With SOD And GPX Activities

Glutathione peroxidase (GPX) and superoxide dismutase (SOD) are the antioxidases in the organisms. GPX is a kind of selenium-containing enzyme. It distributes extensively in cell, blood and tissues. GPXs prevent organism from damage of free radicals by scavenging hydrogen peroxide and lipid hydroperoxide and maintain normally physiological functions of the cells and tissues. And SODs are also a kind of metallic enzymes, which distribute extensively in the tissues of both aerobes and aero tolerant organisms. SODs are classified into CuZn-SOD, Mn-SOD and Fe-SOD according to the different metal-containing. All the SODs catalyse O2-dismutation reaction. Modern medicine studies on reactive oxygen species (ROS) showed that the metabolic disorder of free radicals is believed to cause many disease, such as cataract, diabetes, Keshan disease and some cardiovascular disease. Thus it is important for drug development studies on both GPX and SOD. Our group has successively synthesized a series of GPX mimics and SOD mimics since the end of 1980s'. We link the N-end of the 17-peptide SOD mimic and the C-end of the 15-peptide GPX mimic, hoping to obtain a new mimic which has both SOD's and GPX's activity. On the basis of previous work, following researches were carried out. 1. The design of the small peptide mimic We linked the N-end of the 17-peptide SOD mimic and the C-end of the 15-peptide GPX mimic, hoping to obtain a new mimic which has both...