The Relationship Between The Biotic And Ecological Factors And Podophyllotoxin Content In Sabina Vulgaris Ant

The evergreen prostrate shrub Sabina vulgaris Ant. (syn.: Juniperus sabina Linnaeus) belonging to the genus Sabina ( family Cupressaceae) is widely distributed in the northwest of China. Much attention has been paid to chemical constituents, bioactives, biotic, ecological and physiological characteristics of this plant. One type of mainly secondary metabolites of the plant, podophyllotoxin and its congeners exhibit pronounced biological activity mainly as strong antiviral agents, antineoplastic drugs, and as insecticides. However, the influences of ontogeny and environment on the content of podophyllotoxin in S. vulgaris still remain unclear. In order to provid scientific basis for the further rational utilization of the S. vulgar and podophyllotoxin., therefore, in this paper the relationship between the biotic and ecological factors and the podophyllotoxin content in the plant were studied, using supercritical fluid extraction (SFE) and high performance chromatography (HPLC), and the results were described as following: 1. The conditions for extraction of podophyllotoxin from S. vulgaris with SFE were studied. The podophyllotoxin content was analyzed by HPLC with PAD detector. The extracting efficiency was evaluated by 2 indexes, i.e., total compound extraction ratio and podophyllotoxin extraction ratio. Using static extraction followed by dynamic extraction methods, the effects of different parameters, such as pressure, temperature, modifier volume and extraction time, on the supercritical fluid extraction (SFE) of podophyllotoxin were investigated. The results showed that, under a pressure of 6000 psi, temperature 35℃, flow-rate of CO2 40mL/g, and static extraction time of 15 min, extraction was more selective for the podophyllotoxin. 2. The podophyllotoxin content of S. vulgaris leaves and stems in 7 different habitats were examined. The results showed that the habitats exhibited significant influences on the content of podophyllotoxin in the leaves, while little influences on the content of podophyllotoxin in the stems, and the content of the podophyllotoxin of the plant was more higher in protruding lands while less lower in artificial woods 3. The mineral substances in soil of settled sands, protruding land and artificial woods were surveyed respectively, and there was a strong linear relationship between nitrogen and soil-available nitrogen and Fe contents and podophyllotoxin content, and increased nitrogen and Fe content in the soil resulted in increase of podophyllotoxin content. 4. The content of the podophyllotoxin of S.vulgaris leaves and stems in different ages were studied. It was founded that there were notable differences in the content of podophyllotoxin among stems and leaves of different ages, and the contents were decreased in the following order: annual leaves > biennial leaves > annual stems > biennial stems > triennial stems, On the basis of these facts it may be concluded that the leaves and the younger tissues of S.vulgaris were rich in podophyllotoxin. 5. A comparison of podophyllotoxin content among 2 differences species showed that the content of podophyllotoxin among Sabina rulgaris Ant.Var.yulinensis was higher than that among Sabina rulgaris Ant.obviously. 6. The content of the podophyllotoxin of S.vulgaris in immature seeds and seeds were analyzed. The podophyllotoxin was found in higher content in immature seeds and in lower amount in seeds 7. The results also showed that there was notable variability in S.vulgaris with respect to podophyllotoxin content all year long. The content of the podophyllotoxin was relative higher during April, July and November while relative lower during June and August. It appears that the accumulation or production of podophyllotoxin in S.vulgaris was mainly during April, July and August in the year.