| The investigation studyed the effect of Berberine, Baicalin and Astragalus Polysacharin using the two-cell embryos of ICR mouse as explant. Three experiments were set: the effect of Berberine, Baicalin and Astragalus Polysacharin on Two-cell Embryos Development in vitro; the effect of Berberine, Baicalin and Astragalus Polysacharin on the cell numbers from development to hatched ; the effect of Berberine, Baicalin and Astragalus Polysacharin on embryos earlier transplant . Experiment 1 used the mCZB as control, supplemented with Berberine, Baicalin and Astragalus Polysacharin respectively. After several replicated, 4-5 concentraitons were selected to further selection. The result showed that the best concentration of Berberine is 0.10ug/ml which 120h embryo incubated rate of 89.9% was much higher than the control(50.7%), there was no big difference group; The mCZB supplemented with Berberine,Baicalin and Astragalus Polysacharin significant improved the development of two-cell embryo in vitro. Experiment 2 compared the number of incubated embryos under different concentrion of Berberine, Baicalin and Astragalus Polysacharin with control using the incubated embryos of emperiment 1.the number of embryos supplemented with different concentration of Berberine(83.7 ±9.10) were much higher than the control(69.5 ±7.14).there was no difference between the mCZB supplemented with Baicalin or Astragalus Polysacharin with the control. The result showed that Berberine effectively improved the number of embryos in vivo. Experiment 3 used the antibiotics mCZB as the control, supplemented with Baicalin and Astragalus Polysacharin respectively. The two-cell embryos were transplanted after blast in vitro to study the effect of Berberine, Baicalin and Astragalus Polysacharin on transplant efficiency and reproduction. The results showed that Berberine and Baicalin improved the embryos'development and replication in vivo, Berberine, Baicalin had no harm to embryos transplant and birth mouse.
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