Proteomic Analysis Of The Effect Of Pulsed Electric Field On The Protein Expression Of Suspension Cultures Of Taxus Chinensis

A significant increase in intracellular accumulation of taxuyunnanine c (Tc) was observed by exposing the suspension cultures of Taxus chinensis in early exponential growth phase to a 30-min pulsed electric field (PEF, 50Hz, ≈10V/m) in former study. Proteomic analysis was used to research the changes of the protein expression of suspension culture of Taxus chinensis 48 hours after the pulsed electric field elicitation. The two dimensional gel electrophoresis (2-DE) experiments was repeated for 3 times, and proved to be reliable according to the correlation coefficient analysis. Distinct changes were found from comparison of the protein patterns of control and PEF treated cells. Matrix-assisted laser desorption/ionization mass spectrometry and electrospray ionization mass spectrometry were used to analyse some changed proteins. The conclusions indicated that heterogeneous nuclear ribonucleoprotein(hnRNP), aldose 1-epimerase family protein and Ferredoxin—NADP(+) reductase, putative/adrenodoxin reductase were related to the induction of PEF.