Studies On Phylogeny And Their Photosynthetic Characteristic Of Tai-zi Lotus, Aether Lotus And Present- Lotus

It is considered that lotus (Nelumbo nucifera Gaertn.) is one of the pioneer angiosperm, which shows both characteristic of dicotyledon and monocotyledon and has the most important place in the evolvement of angiosperm system. The lotus can produce chlorophyll at condition of invisible light, which is rarely for higher plant; On the other hand, the lotus is famous for its longevity, which can grow into spring seedling even though it was buried underground for hundreds of years and even thousands of years; The particularity of lotus spurs domestic and foreign researchers to research them.DNA polymorphism of Tai-zi lotus, Aether lotus and three kinds of present lotus is studied by AFLP analysis. 56 selective primers produced 1445 polymorphic bands out of the total of 2550 bands amplified, accounting for 56.7 %. The molecular phylogenetic tree generated by UPMGA method showed that the genetic relationship among 5 accessions, they can be grouped into two groups as Nelumbo Lutea(wild.) and Asia Lotus when the similarity coefficient is 0.63 and grouped into three groups as Nelumbo Lutea(wild.), Asia Lotus and cross bred by Nelumbo Lutea(wild.) and Asia Lotus; The above results are consistent with the past research conclusion.Then the photosynthetic characteristic of the five samples are studied on structure and function, and effect of temporal and spatial on the photosynthetic characteristic are also discussed. Chloroplast structure of the samples are observed by light microscope and electron microscope and results show the chloroplast ultrastructure are similar among Tai-Zi, Nelumbo Lutea(wild.), peony lotus and Wu-fei Lotus, having model morphologic characteristic of higher plant and there obviously have substratum and granulum basale, but compared with other present lotus, chloroplast of Aether lotus has not obvious substratum and granulum basale. The study of the function indicates that the chlorophyll content of Nelumbo Z,wtea(wild.) is the highest which shows there are tiny difference in chlorophyll although Nelumbo Lutea(wild.) and Asia Lotus are similar in configuration;, chlorophyll content of Aether lotus is consistent with two present lotus; chlorophyll content of Tai-Zi obviously decrease compared with two present lotus. Fluorescencespectrum indicates the active of light system II of Aether lotus slowdown compared with present lotus, which are influenced by outer space microgravity and ray.In this paper, the results of genealogy are confirmed by results of genetic relationship among five group lotuses. The study shows that the photosynthetic characteristic of Tai-zi lotus are similar with present lotus; while chloroplast structure and function of Aether lotus are changed, which shows photosynthetic system II decrease.