Sequencing And Analysis Of The ITS Sequences From 10 Isolates Of Alexandrium

In this research, the ITS(Internal Transcribed Spacer, ITS) regions including 5.8s rDNA from ten isolates of Alexandrium(from Donghai of China, Dapeng Bay of China, HongKong, Taiwan Province et al.) were amplified by polymerase chain reaction (PCR). The ITS fragments were sequenced successfully. With another 11 choosen ITS sequences from GenBank, we calculated the Knuc values and constructed phylogenetic tree by Maximum Parsimony methods. The results showed the relationship of the isolates. And we found the isolate A.minutum AM may be identified incorrectly because it was more like A.tamarense than A.minutum. During the experiments, a new method of ten-minute pre-dealing of templates before PCR was used which saved a lot of time and greatly reduced the expense.