The Pilot Study Of Monascus Mycelium's Growth Mechanism And Its Chemotaxis Growth

The ramification or divarication mechanisms of filar microbe as soon as its self-control mechanism are focus of study to microbe scientists all though. Combine with predecessors' mature theories and upon observed phenomenon, the author setup many hypothesis and some model for the Monascus' growth in this paper. Moreover, the reason of Monascus' chemotaxis growth tried to be explained. There are some models as followed:1. The water permeability mechanism in Monascus' solid cultivation. The surrounding's humidity has great effect on the ferment of the Monascus and its modality, which has been relatively discussed less up to date. According to the observation of the Monascus modality with solid state culture, combining simple diffusion with active transportation a permeable model for the water's transportation is set up: the water molecules can pass phospholipid bilayer by simple diffusion from the high flow to the low flow. The changes of the surroundings humidity arise the changes of the flow's direction and velocity. On the other hand, active transportation is lagged more and wastes more energy compared with the simple diffusion. The paper analyzes that on the active state the active transportation gains higher efficiency than the simple diffusion and the both function at the same time can determine the Monascus mycelium's configuration and its fermentation capability. With the flow of water, the threshold value of the surroundings humidity has been deduced as RH75%. Moreover, following experiment has validated the result that the Monascus' mycelium cannot grow naturally until the surroundings humidity arrives at the value.2. The 'Ramification-Two-Threshold-Model' of Monascus. When the concentration of the surroundings infection gene is lower than Ramification-Inhibition-Threshold (RIT) t₁, the mycelium could divaricated;on the other hand, when the concentration of nutrition is higher than RIT t2, the mycelium could divaricated without inhibition and spread around sufficiently.3. The " of Monascus. When the nutrition concentration in the mycelium reach the Growth-Threshold T1, the grow speed of the lawn will be invariableness and the increasing area of the lawn are both invariableness;when the mycelium's nutrition concentration are higher enough thanInhibition-Threshold T2, the increasing area of the lawn will be lowed because of the surrounding's high infiltrate pressure.4. Different attractants do different effects on the Monascus' chemotaxis growth as soon as different surroundings.What's more, two Graphic User Interfaces (GUI) were designed to calculate colony areas using MATLAB. It is convenient and easy for users to calculate the irregular colony areas of filamentous microorganisms.