A Design Of The Fluorescent Microscopy System To Measure [Ca～(2＋)]_i In Living Cell

In order to detect [Ca2+]i, a novel fluorescent microscopy system to detect [Ca2+]i in living cell has been designed. The main work is divided into two sections: the design for monochromator and the design for the system to detect the faint fluorescent signal.In the system of monochromator, the light from the short-arc xenon lamp is first converged by the ellipse mirror, then collimated by paraboloidal mirror, and diffracted by the grating, at last focused into the fiber by triple-lens whose another end export the monochromatic wave. In the design of this system, the optical axis redirection method is employed, efficiently reducing the noise of the lamp, and this system uses the economical ellipse mirror,not the torodial mirror. In this equipment to detect the faint fluorescent signal, the weak fluorescence emitted from fluorescent probes is collected by the PMT, converted to electronic signal that is then amplified and filtered by the amplifier and Bessel filter.The main content of the paper is divided to three sections as follows: The first part concerns the structure and design of the monochromator and the rule of controlling wavelength. The emphases in this part is the design of the system to disperse the light. The second part introduces the design for the system to detect the faint fluorescent signal. In the system, we use PMT to detect the fluorescent signal. The last part particularly describes and the process including system installation and wavelength calibration. Then, using this system, we get the satisfactional experiment results .