The Delta Opioid Receptor Activation-Induced β-arrestin 1 Nuclear Translocation And Its Interaction With P300

β-arrestin1 (β-arr1), an important cytosolic regulator of G protein-coupled receptor (GPCR) signaling and scaffold, is known to distribute evenly in the nucleus and cytoplasm, however in the nucleus its function(s) remains unknown. In this study, we showed that stimulation of delta-opioid receptor resulted in the translocation of βarr1 from the cytoplasm to the nucleus, and β-arr1 could interact with p300, a histone acetyltransferase (HAT), in the nucleus. The main assays performed in the study are listed as below: nuclear extract preparation, immunoprecipitation assay, immuno-fluorescence assay, western blotting, deletion construction and mass spectrometry. In HeLa and SK cells, stimulation of delta-opioid receptor or stimulation by PMA could lead to the translocation of βarr1 from cytoplasm to the nucleus; in contrast, stimulation of kappa-opioid receptor or beta2 angiotensin receptor could not. Using immunoprecipitation and immuno-fluorescence assays, it was demonstrated that β-arr1 could interact with p300 in the nucleus. Furthermore, stimulation of delta-opioid receptor could enhance the interaction between β-arr1 and...