Molecular Phylogeny Of Morchella And Related Genus

Morchella belongs to Morchellaceae, Pezizales ,Ascomycota. They were first embodied in Ben Cao Gang Mu, writing by Li Shizhen, and now proved to be rare wilding fungus and famous for the outstanding value both in pHarmacology medical uses and edibleness.Although a lot of elaborated researches have been carried out by mycologists all over the world, the systematics of Morchella category is still a controversial issue. With the newest developments in molecule biology, using data of big biology molecule especially DNA sequence analysis, play an important role in investigating and revealing the phylogenesis of fungus. Meanwhile, it also provide the possibility of explaining the phylogenesis of morels at the molecular level, basing on efficient supply from the data evidence we have acquired. In this research, 20 ITS sequences belong to 6 species of the Morchella have been tested. The ITS sequence acquired from the test and the sequence acquired from GenBank using as outgroup (Verpa, Disciotis) are analyzed with Maximum-Parsimony method(MP) and Neighbor-Joining method(NJ). Then a phylogenetic trees has developed in order to demonstrate the phylogenetic relationship between Morchella and related genera.The research shows that there are two main branches in Morchella according to the ITS sequence analysis, black morels and yellow morels. Black morels includes M.angusticeps,M.costata, M.elata, M.conica, yellow morels includes M.esculenta, M.crassipes. This taxonomy and "species aggregate"concept are anastomose in modality.Noticeably, M.conica is differ from the others obviously. And the proleptic environmental affections on phylogenesis of morels are proved by diversities presented in the same species in different areas in their genetic and phylogenetic. Consequently, this research provides a solid molecular character evidence in the systematic study on Morchella.