Construction Genomic Library Of Extremely Thermophilic Anaerobic Bacterium BANG 2(Cadicellulo Siruptor BANG 2) And Cloning Of Endoglucanase Gene Fragments

Carbohydrate is the most widely distributed carbohydrate in the world.The utilization and transformation of carbohydrate may contribute a lot to the human being. It is significative to do some study on carbohydrate.An extremely thermophilic anaerobic bacterium was isolated from BangNazhang hotspnng in Yunnan province, named Bang2. The strain Bang2 was initially identified as Cadicellulo siruptor.Genomic DNA of Bang2 was extracted and was partially digested by restriction enzyme EcoRl, and ligated with plasmid pUC18 by T4DNA ligase. After transformation E.coli JM109,the genomic library was constructed and 6.3 × 103 recombinants was obtained. The ratio of the recombinants which formed transparent zones on the Congo Red plates was 23.5%. The results after digested by restriction enzyme EcoRl revealed that the recombinants which had been picked out contained foreign DNA fragments. The results showed that the endoglucanase gene has been cloned. No plasmid was detected. Under heat induced (37℃) , the expression recombinant protein was analyzed by SDS-PAGE and its relative molecular mass is 46kD. Our results may lay a foundation for the further exploitation and utilizationof cellulose.