| The Comet assay, also known as the single cell gel electrophoresis (SCGE) assay, is a rapid, visual, and highly sensitive technique for measuring DNA breakage in individual cells. The technique is rapidly becoming a widely used analytical procedure because of its unique advantages. The objective of this study was to adapt the Comet Assay tech-nique to the test conditions of our laboratory. In this study, CHL cells were treated for 2 h with K2Cr2O7 solution at five different concentrations (12.5, 25, 50, 100, and 500 μmol/L). DNA damage was then determined using the Comet assay. Comet graphs of the cells together with associated data indicated that comet tail length increased as K2Cr2O7 concentration increased, thereby showing a dose-response relationship. Thus, the Comet assay was successfully adapted to the conditions of our laboratory. Optimum treatment conditions included the following: optimum conditions of slide preparation ; CHL cell treatment period at least 2 hr; CHL cell lysis period of 2 to 2.5 h; alkali unwinding period of 30 min; electrophoresis conducted for 20 to 30 min at 25 V and 300 mA.
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