| Celastrus angulatus M. is a defoliated vine and used to be as traditional Chinese herbs. C. angulatus is also the valuable resources to be made of biorational pesticide. More than twenty secondary metabolites have being isolated from its bark and seed, and the most of it is the active substance. C. angulatus has a significant and bright prospects of development, so making efforts on this field is very important.The technologies of plant tissue culture has its unique place in biotechnology, and study of tissue culture and plant regeneration in C. anyulatus M. had significant theoretical meaning and applied value. The main contents and results of research in this thesis are as follows:Firstly, Study of tissue culture and plant regeneration in C. anyulatus: The MS medium containing 2,4-D2mg/L is suitable for the induction of callus. The MS medium, which contained 6-BA2mg/L and NAA0.2mg/L, is suitable for shoots differentiating from the callus. The MS medium, which contained 6-BA2mg/L and NAA0.2mg/L, is suitable for shoots growing. The MS medium with MET0.7mg/Land IBA0.5mg/L , darkness and 1/2MS medium were advantageous for the roots occurred. The survival rate of plantlet was 85% after transplanting under suitable condition.Secondly, the ultrastructure of leaf cells in albino seedlings, etionlated seedlings and normal seedlings of C. anyulatus was observed by ultrathin-section and electron microscopy techniques. The results showed that there were distinct differences among ultrastructure of the leaf cells in albino seedlings, etionlated seedlings and normal seedlings of C. anyulatus. The cytoplasma amount in the most cells of etionlated seedlings was few. There were few chloroplasts and the conformation of chloroplast varied from each other in the albino seedlings leaf cells. Few chloroplasts were normal and the most presented part degeneration. Some chloroplast inalbino seedlings took on the state that part membranes were fusing. There were little difference on cell wall, and cell form among albino seedlings, etionlated seedlings and normal ones.Thirdly, the anatomical observation on adventitious root of C. anyulatus vitro was carried by means of paraffin section method. It is significantly clear that adventitious root primordium has not been found in the stem. The adventitious root primordium of stem initialed from the vascular cambium or phloem parenchyma cell.The effect of environmental stresses on the growth and development of plant cells and tissues is one of important fields in plant physiology. Ultrasound as one kind of environmental stresses, it has been found that can greatly influence the growth and development of plant. Where as, the studies about the effect of ultrasound stimulation on vitro is rare so far. In this thesis, the biology effect of ultrasound on C. anyulatus vitro was primarily explored. The results of research in this thesis are as follows:Firstly, when C. angulatus vitro were treated by Ultrasound with a certain intensity of 250w and frequency of 27KHz froml minute to7 minutes, the adventitious shoots from C. angulatus vitro can grow effectively. But treated by Ultrasound with a certain intensity of 500W and frequency of 1.1 MHz , the growth of the adventitious shoots were inhibited in initial stage.Secondly, ultrastructure of the leaf cells of C. angulatus stimulated with 27KHz ultrasonic in different time was observed by scanning electron microscope and transmission electron microscope. The result showed that the stomas in the affected surface were more opened than in the surface of normal leaves. From 1 minute to 5 minutes, the stimulated time more long, the stomas were more opened. But when stimulated from 7 minutes to llminutes, the degree of stomas opened was minish. The ultrasonic also scathed cell membrance, cytoplasm and nucleus. With stimulated longer, the cells were injured greatly.Thirdly, the effect of ultrasonic stimulation on protective enzymes ofCelastrus angulatus vitro was explored. When Celastrus angulatus vitro were treated by Ultrasound with a certai...
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