| The addition of elicitor is a effectual method to improve the production of secondary metabolite in plant cell culture. This paper studied on the interaction of elicitor with Catharanthurs roseus Cell and discussed the effect of elicitor on the alkaloid metabolism, of Catharanthurs roseus Cells in suspension culture the results as follow:The alkaloid biosynthesis in Catharanthurs roseus Cells in suspension culture was enhanced by treating with five kindsof fungal elicitors separately ,among them the Aspergillus niger elicitor had the highest inducing efficiency, The alkaloid yield was 1.6 folds of the control,reaching to 666ug /gFW. The best response of Catharanthurs roseus Cells to elicitor treatment was in the late exponential Growth phase.Effects of fungal elicitor on cell redox status and alkaloid production were studied in suspension cultures of Catharanthurs roseus in the late exponential stage . the results show that fungal elicitor induced oxygen burst, changes of cell redox status , alka-linization of medium and fluctuation of the activity of redox enzymes with a sequence. The content of MDA and the content of protein representing the quantity of enzymes increased.The activity of POD could be kept at a higher level in contrast to the control.The activity of CAT was inhibited at first by fungal elicitor and followed by an obvious increased, while the activity of PAL was promoted.The paper showed that after fungal elicitation , the addition of 200mmol/L CaCl2 could furthermore strengthen the elicitation effect, and get more alkaloid production than fungal elicitation;While the addition of 10mmol/L EGTA or 1mmol/L LaCl3 after fungal elicitation restrained the elicitation effectsThe activity of peroxidase (POD), phenylalanine ammonia-lyase (PAL) the content of hydrogen peroxide (H2O2) and the biosynthesis of alkaloid in Catharanthurs roseus suspension culture exposed to various concentrations of salicylic acid was investigated. The results indicated that the addition of SA was followed by an increase in POD, PAL activity and it also promoted the content of H2O2 and alkaloid, alkaloid productivity increased 1. 3-fold when the cultures were exposed to 10mg/L SA. It suggested that SA couldbe used as an elicitor to improve the alkaloid in catharanthurs roseus cell culture.Effects of the treatments of 300ug/mL fungal elicitor (FO), 10mg/L salicylic acid(SA) and 300ug/mL F0+10mg/L SA on membrane -lipid peroxidat-ion and alkaloid production in catharanthurs roseussusupension cell cultures were studied . the results showd either FO or SA caused cell to membrane-1ipid peroxidation . combined treatment of SA and FO could decrease the degree of cell membrane-lipid peroxidation caused by only FO treament .combined treatment induced higher PAL,POD activity and obtained more biomass than that of only FO treament did. Among three treatments, the combined treatment get the most alkaloid production. The results suggested that combinedof FO and SA obviously improving alkaloid production be possibly related to addition of SA relieving membrane-lipid peroxidation induced by treatment of fungal elicitor.
|
PDF Full Text Request