Study On Environment Control Combined With Clean Technology In A Closed Tissue Culture System Under Artificial Lighting

As a new technique for tissue culture, photoautotrophic micropropagation has many advantages such as promoting growth and development of leafy explants, and minimizing microbial contamination. It will be widely used for the large-scale plantlets production in the near future. The rapid development of the photoautotrophic micropropagation in China requires integrated environment control and air purification simultaneously. In this study, an environmental control system combined with clean technology was developed to control air condition and lighting environment in a closed tissue culture system under artificial lighting. The experiment results are shown as following.1. The environmental control system could achieve to control temperature, relative humidity, CO2 concentration, light intensity, and cleanliness, simultaneously.2. The control point showed an uniformity in themperature, relative humidity, CO2 concentration within ±0.4℃,±7.9%, ±34 mol mol-1, respectively. The gradient of temperature and relative humidity were 2.3 ℃ and 4.0% in the closed tissue culture system when 1/4 and 1/2 of all fluorescent lamps were turn on. However, when all of the fluorescent lamps were turn on, the gradient of temperature was 3.9℃ and relative humidity was 65.0±17.5% even the setpoint was 65.0±3.0%. Before the system running and after one year, the cleanliness measured by airborne particles and settling microbe were both 7 grade according to GB50073-2001 and GB/T 16294-1996.3. Based on theoretical analysis and experimental results, the high relative humidity was diffcult to maintain in the closed tissue culture system when all of the fluorescent lamps were turn on, because of deficiency of the humidifier's capacity and air ventilation by top air nozzle outlets and return air in the bottom.4. The ginger (Zingiber officinale) plantlets cultured in triangle vessels under photoautotrophic micropropagation were vigorous and 1.4, 1.5, 1.7 times in leaf number, dry weight, and stem elongation compared to those culutred under conventional micropropagation, respectively.5. Some improved suggestions were shown based on the disadvantages of the environmental control system in the closed tissue culture system.