Genetic Diversity Studies Of Cymbidium And Dendrobium Based On RAPD And AFLP

In this study, the genetic variations of 14 species of Cymbidium were revealed by using RAPD and AFLP markers and the genetic variations of 13 species of Dendrobium were revealed by using AFLP markers.Twelve primers selected from 100 random primers amplified 254 frangments in 14 species of Cymbidium, in which 243 segments are polymorphic. The rate of polymorphism was 95.7%. And all the segments were ranging from 200bp to 2200bp. The cluster analysis with UPGMA suggested that the tested materials were clustered into two groups on coefficient level 0.46. Group I included C.qiubeiense, C.goeringii var. longibracteatum, C.goeringii, C.faberi var. omeiense, C.kanra,n C.nanulum, C.sinense,C.ensifolium. Group II included C. lancifolium, C.mastersii, C.eburneum, C.wenshanense, C.hookerianum, C.iridioides.Three selective primer combinations were used to amplify in 14 species of Cymbidium, which were E+ACT/M+CAA, E+ACT/M+CAC, E+ACT/M+CAG. Ranging from 100bp to 300bp, a total of 279 AFLP markers were produced, among which 223 polymorphic bands werepolymorphic. The polymorphism rate was 79.9%. The cluster analysis with UPGMA indicated that the 14 species were also clustered into two groups on coefficient level 0.72. Group I included C. lancifolium, C. qiubeiense, C. goeringii var. longibracteatum, C. goeringii, C. faberi var. omeiense, C. kanran, C. nanulum, C. sinense, C. ensifolium. Group II included C. mastersii, C. eburneum, C. wenshanense, C. hookerianum, C. iridioides.The results of UPGMA cluster analysis obtained from RAPD data and AFLP data were in essence consistent, and also very closed to Du Puy & Cribb morphological classification. Two results showed that the genetic distances are closest between C. ensifolium and C. sinense, C. kanran andC. faberi var. omeiense, C. mastersii and C. eburneum.Three selective primer combinations were used to detect the genetic variations in 13 species of Dendrobium , which were E+ACT/M+CAC,E +AAC/M+CAC,E+ACA/M+CAC.Abundant bands were gotten. Between lOObp and 300bp, a total of 346 DNA bands were amplified, 342 of which were polymorphic. The rate of polymorphism was 98.8%. The cluster analysis with UPGMA indicated that the 13 species were clustered into four groups on coefficient level 0.56. Group I included D. falconeri, D. officinale, D. wilsonii, D. heterocarpum, D. crystallinum. Group II includedD. hancockii, D. guangxiense, D. primulinum, D. crepidatum, D. thyrsiflorum. Group III included D. chrysotoxum. Group IV included D.loddigesii. The results were different from that of morphological study, indicating that classification of Dendrobium remain to study further.