Preliminary Study On Low-density Microarrays For Polypeptide: N-acetylgalactosaminyltransferases Gene Family Expression Profiling

Objective To analyze the differences of N-acetylgalactosaminyltransferases gene expression profile in different human tumor cells with preliminarily made cDNA microarrays. Methods Mainly by means of PCR, TOPO-TA cloning and molecular hybridization techniques. Results N-acetylgalactosaminyltransferases cDNA library has been made successfully for the creation of low-density microarrays for gene expression profiling , with which , the different positive signals of N-acetylgalactosaminyltransferases gene expression can be observed in human tumor cells through chemiluminescent detection. Among the signals, those of pp-GalNAc-T2 and pp-GalNAc-T9, T13 gene expression are the highest in human gastric cancer cells and neuroglioma cells, respectively. The results show that , (1) in different human tumor cells , N-acetylgalactosaminyltransferases gene expression levels differ from each other. (2) in the same tumor cells, each N-acetylgalactosaminyltransferases gene which differs in expression levels, displays noticeable differences from those in normal cells. Conclusion N-acetylgalactosaminyltransferases genes have different expression profiles in different tumor cells. At the sametime, it also preliminarily proves that there is a certain relation between N-acetylgalactosaminyltransferases gene expression levels and tumor formation, which necessitates more detailed studies.