1.Tandem Mass Spectrometry Application On Proteomics 2.The Structure And Function Studies Of JingZhaotoxin-Ⅶ

Part one: Tandem mass spectrometry is impertinent to studying of protein and proteimics . Its application would be founded on two way in this article: (1)on de novo sequence: two natural peptides whose sequences are partly unknown through Edman Degration were analysised by de novo sequence method of nano-ESI-MSMS. Results indicated that this method can effectively solve the difficulty occurring in Edman Degration , such as losing of the last residue, unidentification between leucine and lysine residues. The established methoed is a good supplement for Edman Degration. (2)on analyzing the proteins in SDS-PAGE: The p53 related proteins from HNE of nasopharyngeal carcinoma cell was subjected to In-gel enzymatic digestion after SDS-PAGE .The tryptic peptides were fragmented using capillaryHPLC-ESI-Q-TOF to produce a series of MS/MS spectra. Throught Mascot database search, nine proteins were characteristiced to related with p53.