| Platycodon grandiflorum (Jacq.) A. DC. (P. glaucum Nakai)is a kind of commonly used important traditional Chinese medicine, Perennial herb, which belongs to Campanulaceae and whose root is used as medicine of eliminating the phlegm, arranging efficiency, moistening the lung , breaking up suppuration and so on. It contains kinds of amino acid and essential trace elements that the human body needs. And it contains too a great quantity of unsaturated fat acid such as a large amount of inferior oleic acid, etc., which can reduce blood pressure and grease, resist scleratheroma and other kinds of function. The experiment trains systematic research to Platycodon grandiflorum, its tissue culture and plant regeneration system have been established and perfected. Moreover, such physiology activation and accumulation situation of valid components have been researched in liquid medium. And technology of total saponin from Platycodon grandiflorum and the concentration of antibiotic in course of trans-genes are chosen out in the preliminary research. The main results of the experiment are as follows:1. With the explants of the leaf and stem, terms of induction of callus, adventitious buds, proliferation of buds and rooting are selected out, And relatively simple and feasible Platycodon grandiflorum tissue culture and plant regeneration system is established: The medium of MS+2,4-D 2mg/L + 6-BA 0.5mg/L + NAA Img/L +IAA 0.5mg/L can induct leaf-callus, induction rate is 95.9%; The medium of MS+2,4-D Img/L + 6-BA 1.5mg/L + NAA 0.2mg/L +IAA 0.5mg/L can induct stem-callus, induction rate is 92.8%. Moreover, anti-salt quality of callus has been explored initially.2. The hyperplasia, physiology activation and accumulation situation of valid components of callus of Platycodon grandiflorum in liquid foster have been measured. Utilizing the callus cells in large-scale production of valid components will remain and research further.3. The technology and solvent of total saponin from Platycodon grandiflorum have been optimized. Moreover, the results have been verified by the way of slight layer. In conclusion, the best means is abstracted 60 minutes with the supersound of methyl alcohol.4. At the same time, in three main origin place of the boundary of the Shaanxi, content of total saponin has been compared. Shangluo's is slightly higher than two other places.5. Utilizing the leaf and stem ofPlatycodon grandiflorum as explantss, effects ofdifferent antibiotic to induction and propagation of callus are researched. It shows: Inhibition to induction and propagation of callus in different degrees from Kanamycin and to induction of buds from ceftriaxone sodium has been found; Different explants confronted with antibiotic have different sensitivity: Leaf has higher sensitiveness than stem to kanamycin, a section of sensitivity of ceftriaxone sodium of blade and stem is nearly unanimous.6. The suitable concentrations of antibiotic used in the trans-genes have been selected out, conclusions are: When transforming with callus as explants, concentration of selective factor should be set for 25 mg/L Kanamycin; When transforming with stem and leaf as explants inducted callus, concentration of choose-factor should be set for 50mg/L and 25 mg/L Kanamycin separately; When transforming with them as explants inducted adventitious buds, concentration of selective factor should be set for 50mg/L and 25 mg/L Kanamycin separately;When restraining fungus, concentration of ceftriaxone sodium is proposed and settled as 400 mg/L.Cornus officinalis Sieb.Et Zucc is a common and rare traditional Chinese herbal drug, deciduous tree, which belongs to Cornaceae , whose dry drupe is used as medicine and has extremely high medical value, such as dropping blood sugar, anti-ageing function, strengthening the immunity function ,being used for treating insomnia, diabetes and other inflammations. Investigation and Exploitation of existing Cornus officinalis Sieb.Et Zucc plant resource state are systematically surveyed in the boundary of distinct original...
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