| In the development of oocyte, pro-oncogene, sex steroids and their receptors play important roles. Rana temporia chensinesis were sampled in this study. By using histological method, the thesis studied the period of follicle development of the Rana temporia chensinesis. By using immunocytochemical method, regulations of estradiol, progesterone, testosterone, and their receptors, c-myc, c-fos, c-myb were under our research during follicle development of Rana temporia chensinesis. Studies on the regulations of steroids, receptors and proto-oncogenes during the follicle development, and researches on these mutual relations, have great significance in the study on the mechanism of the oocyte development. The results are as follows.1. From Apr to Jul, the ovary index was low. Since Aug, it began to ascend. It reached its peak on Mar next year. According to the classification of Chester to amphibian follicals, the development periods of Rana temporia chensinesis were divided into four parts: stage I: primordial follicles, very small, mostly formed in Apr; stage II: previtellogenic follicles, mostly formed from May to Jul; stage III: vitellogenic follicles, mostly formed in Aug; stage IV: postvitellogenic follicles, mostly formed from Sep to Mar next year. Each female only ovulates once every year. For the community that lives in Qinling mountain area, spawning period lasts from Mar to Apr. The little individual difference exists in the ovary of the Rana temporia chensinesis.2. E2 was visualized at stage II and III. The immunostaining for E2 was weak at IV stage. The result of Estrogen receptor basically identified with that of E2. Cytosolic and nuclear Estrogen receptors were measured. The result indicates that Estrogen receptor regulates the development and maturation of oocyte by genomic or nongenomic action. In follicle cells the immunostaining for Estrogen receptor was characterized. It shows that E2, with its receptor, still might self-regulate follicle cells which secret estrogen.3. The immunostaining for P was not found at stage I and II. P increased at stage III and IV. These results indicate P regulates oocyte maturation and control synthesis of vitellogenin. The result of PR identified with that of P. The genomic or non-genomicaction of P regulates the development and maturation of oocyte via cytosolic and nuclear Progesterone receptors.4. T, the precursor of E2, was found a little bit at stage I and II. At stage III and IV the immunostaining for T and AR was strong. It shows that T still might regulate oocyte maturation and inhibit synthesis of vitellogenin. AR was visualized in cytosolic and nuclear. It shows that T regulates the development and maturation of oocyte by genomic or non-genomic action.5. In the development periods of follicles, the results of sex steroids identified with that of their receptors. At stage I and II, when the immunostaining for E2 and ER was strong, P and PR were not found, T and AR were found a little bit. At stage III and IV, the immunostaining for P, PR, T and AR was strong, E2 and ER were found a little bit. These findings show that these three sex steroids can auto-regulate their own receptors respectively and hetero-regulate other receptors. In addition, the three sex steroids were found differently in distinctive development periods, adapting themselves with the hormone demand by follicles development. Whether the difference is formed through mutual conversion in the synthesizing course or mutual control functionally requires further research.6. Myc was visualized in oocyte cytoplasm at stage I and II. It was not found at stage III and IV. It shows Myc maybe plays a role at the early stage of oocyte development. In follicle cells the immunostaining for Myc was characterized. The result of Myc basically identified with E2, and was opposite to that of P.7. The immunostaining for Fos was weak in follicle cells at stage I and II, and was strong at stage III and IV. The result of Fos basically identified with P. It shows Fos maybe regulates synthesis of...
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