The Concentration Technique For Cyanophage PP And Its Physiological Characters Of Multiplication

Concentration is the important prophase step for studying cyanophage. A new concentration system, the backflushing ultrafiltration system , was constructed with the isolated cyanophage PP in our lab associated with the ultrafiltration technique.Basing on this system, the cyanophage concentration multiple could reach up to 30 times and the recovery rate of the cyanophage could exceed 90%. This method was also effective for concentrating virus form large volume water samples. Moreover/the concertration multiple of cyanophage PP could rise to nearly one-hundred times when two-step backflushing ultrafiltration technique was adopted.Again,using cyanophage PP and adopting orthogonal design ,we have \ performed the studies on the effects of several environmental factors on the multiplication of the cyanophage. Results are as follows:In the experiment of measuring the adsorption rate,temperature was found to be the primary influencing factor and displayed an obvious significance by the statistics analysis. As temperature rose, the saturated adsorption rate tended to increase ,and the time to reach saturation would become longer. Comparatively, the sunlight radiation showed a minor influence. With the intensity of sunlight radiation strengthened, the adsorption speed of the cyanophage to the host gradually quickened, and reached the maximum of 62.4% at 28Cand 2000 Lux.Results involved in measuring burst size and latent period of the cyanophage showed that both temperature and sunlight are the important effect factors. Under the dark condition or at low temperatures, the cyanophage wouldn't multiply, but the elevation in both temperature and sunlight would bring about the burst size to increase. The rising temperature would lead the latent period to shorten, though the increasing sunlight intensity did not show much influence. In comparision with temperature and sunlight, the level of N and P concentration as well as the growth phase of the host represented the subordinate effect factors for the multiplicationof cyanophage. In our experiment at 28C and 2000 Lux ,it gave rise to the maximal burst size of 135 PFU cell-1 for cyanophage PP ,when it infected the lag phase host only grown in 2mM N and 0.2mM P medium.Furthermore, by adopting the experimental data and stepwise discriminant analysis,we established the preparatory dynamic balance equation for the change in the population of cyanophage PP in the natural environment as follows: N(t)=N(t-1)+N(t-n)* Y(A)* [ Y(B)-1 ] *dt-N(t)* Y(C)*dt+Y(Dt-1)...