Study On Thermochemical Liquefaction Of Lingocellulosic Wastes And Laboratory Test Fermentation Of Liquefaction Products

Cellulose is macromolecular compound made by more glucose molecules linked through -1,4 indican bond, including 3,500-10,000. It, one of chief components of plant cell wall, lies in the earth abroad. However, cellulose is a most important recycled resources, more abundant and cheaper. With the population increasingly, it is becoming more and more indispensable to energy sources, foodstuff and raw material of industry in future. It's time to be more attention to cellulose.In order to enhance the efficiency of usage of lignocellulosic wastes and protect the balance of ecosystem, the liquefaction of lignocellulosic wastes was studied in this paper. Lignocellulosic wastes and other chemicals were put into the reaction container and then liquefied. The effects of catalysts, solvents, reaction time and temperature on the liquefaction were investigated. The molecular weight distributions of liquefaction products were determined by GPC. In most cases, Low residue content cannot be achieved by single catalyst. However the binary catalysts system favors low residue content. It was found that the residue content were remained at a low level with ethylene glycol as the reaction media and 6%(by mass)of sulfuric acid as the catalyst in the presence of phenol. The contents of lipophilic products were investigated. The minimum residue content was found to be 8.8% and the MW and M of liquefaction products were 806 and 799 respectively. As a result, 1 ipophilic products have the highest yield when the reaction time was 70min. The identification of IR spectrometry showed that there are more dissociated hydroxy (HO-) and end-groups in the liquefaction products than in the plant cellulose. As a result, the crystal structure was destroyed in the thermochemical liquefaction.There are twelve bacteria, conserved five in our laboratory and screened seven through dunghill, putrid garbage or soil. 1 examined decomposition rate of filter paper, CMC enzyme and crude cellulose enzyme of bacteria. Five bacteria, 60.00mg/mL d upwards, were selected. The results show that enzyme is fading away with cellulose content decreasing in the culture media.The liquefaction products were analyzed by applying any analysis instruments including Infrared spectrum (IR) and GC-MS. The results showed that the structure of wheat straw was destroyed in liquefaction, with producing some ramification of glucose Some screened strains can decompose and use the liquefaction products. As a result of destroying crystal of lignocellulosic wastes in the liquefaction, the screened strains can produce pure protein by using liquefaction products, and its yield is 30.74%; the mixed strains can turn the liquefaction products into alcohol, and its highest content is 19.0%(V/V).In the liquefaction of lignocellulosic wastes, there produced gaseous substances as a byproduct. By using GC-MS, the result showed that the gaseous substances are the mixture of organic acids and a compound as a byproduct in the reaction of vitriol oil and phenol. We cultured photosynthesis bacteria (PSB), using trfe mixed substances as an additive of culture medium. Through the analysis of residue of the mixed substances in the culture media and OD660nm of cell concentration, it revealed that cell concentration became higher, and residue content of the mixed substances was lower in a week. We can draw a conclusion that the mixture can promote PSB reproduction and protein content.