| Objective We studied the apoptosis and the DNA damage of Chinese hamster ovary (CHO) cells induced by X-ray and the dose-effect relationship in order to understand the toxicity effect of radiation on reproduction system and provide some valuable data for the genetic effects induced by radiation .Methods CHO cell line was exposed to X-ray of different dose (2-20Gy). The DNA damage effects were determined by 3H-TdR incorporation test. The apoptosis and morphological change of the cell were evaluated using fluorescent microscopy and flow Cytometry analyzer after double staining with Annexin V / IP .Results (1) Rate of incorporating 3H-TdR in DNA of CHO cells was declined with increase of dose of the X-ray and were clearly interrelated at 24 and 48 hours after exposure. The 3H-TdR incorporation and DNA synthesis begin to be progressively restored at 48 hours after exposure(2)The cell apoptosis was observed at 24, 48 and 72 hours after exposure but was more obvious at 48 and 72 hours than at 24 hours. Rate of the apoptosis rose with increase of dose and their peak appeared all at dose of 16Gy.(3)The some morphological change of apoptosis cell, the irregular agglomerate of chromatic pycnosis and periphery distribution ofnucleus and crescent moon of the cell, were observed and small apoptosis body even appeared.Conclusions (1) The DNA damage induced by X - ray rose with increase of dose and positively related.(2)Because of apoptosis resulted from the DNA damage that cannot be repaired, the time of revealing apoptosis is later than one of DNA damage.(3)The apoptosis of cells is an effective mechanism to eliminate actively potential risk in the body and to keep the hereditary stability.
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