Study Of Cloning And Expression Of β Subunit Of Porce Follicle-Stimulating Hormone

Follicle stimulating hormone(FSH) belongs to the family of glycoprotein hormones secreted by the anterior pituitary. Though the content of FSH in pituitary is least and the structure is most unstable, it has very important physiology function.FSH is a heterodimer composed of an a subunit, which is in charge of transcript of signal, and a p subunit, which is the functional subunit and can integrate with the receptor. The recombinant FSH(rFSH) ,which was produced by gene engineered technique, is totally as same as the nature FSH.This thesis is a part of the National Nature Science Fund item 'Study of Gene Expression and Regulate of Porce FSH'. Firstly, the total RNA was extracted from porce pituitary. Then the pFSH β subunit cDNA was synthesis by the Smart' cDNA synthesis kit. The cDNA was then cloned in the chromosome of Pichia Pastoris, which was tested by the Southern-blot.The recombinant was induced to express pFSH β subunit protein, which was tested by SDS-PAGE and Western-blot. It showed that the recombinant can secrete the p subunit which had immunological activity.This technique was reported only by our institution in China. Our study will make the production of rpFSH possible.