A Study On Introduction Of UbiC Gene Into Cabbage Through Agrobacterium-mediated Genetic Transformation

The constuction of plant expression vector,tissue culture of cabbage and gene transformation conditions were studied to obtain ubiC transformants.ubiC gene was obtained from the Escherichia coli.by PCR amplication and was ligated to pUC118 vector. Subsequently, the recombined plasmid was transformed to the strain JM109 of Escherichia coli., which was picked up and was ligated to pBI121. As a result, the plant expression vector of pBI121-ubiC was constructed successfully. The hypocotyl segments of cabbage were used as the explants and cultured on a MS medium supplemented with plant hormone, which was established a cabbage regenerated system.The differentiation rate of adventitious buds was above 80%. In this study,ubiC gene was transformed to cabbage through Agrobacterium mediated system. And kanamycin-resistant callus tissue was obtained by pre-culture, infection co-culture and selection.