The Research Of The Propagation Of Cardamine Hupingshanensis

Cardamine Hupingshanensis, a rare perennial plant in Cmciferae cardamine ,is a new species discovered by Lin-hang Liu and Ke-ming Liu professors of Hunan Normal University . It distributes in Huping mountains in north Hunan province. In the nature , we only found its alimentative reproduction and did not found its sexual reproduction. Its amount and distributive area is very small. Additionally,it is also a kind of delicious wild vegetable and the local people pick it in great deal every tow years. Now, it is in severe danger of annihilation. So, it has great significance to save and exploit this annihilating and delicious plant. The details of this research are expatiated as below: 1 The research about the seed vigor and germination of Cardamine Hupingshanensis.From the researches of the seed vigor measurement and germinating experiment, We-found that the seed vigor is very low tested by paper fluorescent method > BTB method and TTC method, and that the seeds have been doated and dead though we have used several kinds of hormones (eg:GA 2,4-D 6-BA et.),several kinds of pervasive solutions (eg: PEG, Acetone),several kinds of organic and inorganic compounds (vitamin c ,vitamin E,H2O2)and several kinds of inorganic ion ( K+,Ca2+,Zn2+) in the experiments under different temperature and different illuminating conditions .So we think that this group of seed provided by Ke-ming Liu who picked them in the same year have beendead or in deep dormancy.2 The research of the Cardamine Hupingshanensis embryodevelopmentSeeing from the embryo development of Cardamine Hupingshanensis. Cardamine hirsute L.and Brassica campestris L. .though there are some different nuances ,they have same development phases. Their embryo all develop from pro-embryo, heart-shaped embryo, torpedo-shaped embryo to mature embryo, the embryo development of Cardamine Hupingshanesis. is same with that of Cardamine hirsute L. and Brassica campestris L.. Every phase is perfect. The development of the embryo belongs to the Onagrad type and that of the endosperm belongs to the nuclear type.3 The research of the germinating experiments in different seed developing phases and the relationship between the germination of mature seed and surrounding factorsWhen the seed develop to the 30th day, its radicle can grow ,but the whole seed can not become seeding ; To the 35th day ,the seed can become seeding ,but its rate is very low; To the 43th day,the fruit ripe and break, the seed will spring ,and all of these seed can become seeding .The seed has no dormancy, but before the ripe seed germinates it must be activated and the soil must have plenteous water, and the temperature must be kept below 28-30@. In addition, the seed does not need atkelescent surrounding (PH=7-8), and it can germinate well under both illumination and dark conditions.4 The change of the seed vigor in different storing time and under different storing conditions.Cardamine Hupingshanesis seed vigor decreases very fast and the determining factors are the temperature and humidity. Under normal temperature and dry conditions ,the seed longest longevity is 110 days; Under normal temperature and moist conditions, its longest longevity is only 55 days. The finest storing condition is low temperature and dry surrounding. In -20@ and dry surrounding ,after storing 110 days ,the seed vigor index and genninating rate almost do not decrease and the germinating rate can maintain at 80-90% in a long time.5 The researches of seeding planting conditions and its growth regulationThe development regulation in nature condition: In the nature ,Cardamine Hupingshanesis is a kind of perennial plant. It bourgeons in early April,blossoms in later May. And at this time the buds at the foot of the old stem begin to grow .From later June to early July ,its seeds ripe and spring but cannot germinate; At the same time the buds on the branches grow out air-growing roots. From July to Augment ,the branches fall to the ground and its air-growing roots grow will in the earth. The buds on the...