Research Of Some Fluorescent Probes And Its Application In The Determination Of Nucleic Acids

DNA is the main genetic material of the organism. The research about DNA can reveal the magic of the heredity.The investigation on DNA are involving that the determination of the biological samples, detection for the structure of the nucleic acid, and the research about the interaction of the anticancer drug with nucleic acid. For these aims, the fluorescent probes play a important role.So, we focus on the fluorescent probes that can be useful for the determination of the nucleic acid in the biological samples. We hope to find some excellent fluorescent probes to determine nucleic acid. we can do more works about nucleic acid, such as: detecting the sequence of the nucleic acid or knowing more about the interaction of the virus with DNA. Three aspect research have been carried out in this paper: Part 1: The fluorescent enhancement of gallocyanine by nucleic acid in aqueous solution was studied. Gallocyanine and nucleic acid interacted strongly with each other. The excitation wavelength was 239nm and the emission wavelength was 414nm. The intercalation mole was prove to exist. The calibration curve was linear in the range of 0.05~1mg/L and 1~10mg/L for calf thymus DNA. The limit of determination was 0.014mg/L.Part 2: The fluorescence quenching of the 9-methylacridine with the nucleic acid was studied. Studies involving the fsDNA, ssDNA, ctDNA, yRNA, daDNA, revealed that the double-stranded and the single-stranded nucleic acid are capable of quenching the fluorescence of the 9-methylacridine. Base on this, a sensitive determination method of nucleic acid was developed. Under optimal condition, the calibration graphs were between 0.0~2.5 mg/L (fsDNA), 0.1~3.0 mg/L (ssDNA), 0.0~2.5 mg/L(ctDNA), 0.1~3.0 mg/L(yRNA), and the detection limit were 0.027mg/L (fsDNA),0.033 mg/L (ssDNA),0.042 mg/L (ctDNA),0.053 mg/L(yRNA) respectively.Part 3: The fluorescent enhancement of Ni(Phen)2PHPIP2+ by nucleic acid in pH7.3 solution was studied. Ni(Phen)2PHPIP2+ and nucleic acidinteracted strongly with each other. The intercalation mole was prove to exist. he calibration curves were linear in the range of 0.01~0.03mg/L and 0.03~5mg/L for fsDNA. The other calibration curves were linear in the range of 0.01~0.05mg/L and 0.05~5mg/L for ctDNA. The limit of determination was 0.0047mg/L for fsDNA and 0.011mg/L for ctDNA. And the recovery examination of DNA real sample was approved.In the last chapter, we compared our results with other articles, and we found that our results were satisfied.