Studies On Histology And Immunohistochemistry Of Testes In Batrachuperus Tibetanus And Rana Quadranus

Spermatogenesis is effected by many factorS, sex steriod hormone is a key factor in regulating Spermatogenesis. The present work was carried out to study the testicular structure of Batrachuperus tibetanus and Rana quadranus by using histology method in order to discover variation of Spermatogenesis and testicular development during the annual reproductive cycle. To elucidate the relationships among testosterone, 17 3 -estradiol, progesterone three steriod hormones, their receptors and Spermatogenesis, we survey the location of steriod hormone and their receptors by immunocytochemistry for the first time in the testis of two amphbians.We study the roles of proto-oncogene c-fos and c-myc in Spermatogenesis by observing the location of their ontoprotein in the testes of two amphibians.The investigation gets the following conclusions:1. The Spermatogenesis of Batrachuperus tibetanus start in August, in this time spermatogonia begain to proliferate. This proliferation process stop or slow down in winter, and resumes in next March and April, mature sperm appears in August then is stored in testis, and spermiation occurs in the third March and April. It spend 20-21 months from spermatogonial proliferation to spermiation. During this period, sperm live through the winter by storing in lumens at the end of testicular lobular or spermatic duct.2. The obvious individual difference exists in the testis of Rana quadranus. Spermatogenesis start from every June or July, and sperm mature in August or Sebtemper. Matural sperm is stored in the testis until spermiation in the next April or May. From spermatogonial proliferation to spermiation spend 11-12 months. It requires further investigation whether spermatogonial proliferation starts in november.3. Interstitial cells synthesize T in spring and autumn, its spermatogonial proliferation independent on the levels of T. T in the follicular cells in the testis of Rana quadranus regulate sperm maturation by paracrine mechanism. The effects of T on target tissuse are mediated by AR through genomic action. AR in interstitial cells probabaly restrain the steriodogenesis of interstitial cells in-situ. AR play a role inmetamorphoses of spermatid.4. E2 triggers directly spermatogenesis through genomic action and effects the steriodogenesis of interstitial cell by negative feedback via follicular cells. E and ER are possibly related with metamorphoses of spermatid and sperm maturation.5. P and PR probably exert roles during the period of sperm development. P in follicular cells regulates spermiation and sperm maturation. P and PR have relationships with spermatogensis and sperm maturation.6. The product of c-fos translocates from the cytoplasm of spermatogonia to nuclei of spermatogonia, then it induces the transcription of DNA and regulate spermatogonial proliferation and spermatogenesis. C-fos probably regulates the steriodogenesis of interstitial cells directly and effects the communication of information between follicular cells and germocytes. E2 probably induces the activity of c-fos in testis. The concentration of T is correlated with the express of c-fos.1. With testicular development, the products of c-myc translocate from the cytoplasm of spermatogonia to nuclei of spermatogonia. C-myc, as a nuclear transcription factor, can influences spermatogenesis through inducing the transcription of DNA.