Studies On Mutation Of High-Yield Apple Wine Yeast By Ion-Implanting

The wild-type apple wine yeast Y02 was selected as the natural strain, with the treatment of ion-implantation ,the high-yield producing liquor mutant strain was obtained, which was named as ION Vll dry. After fermentation test, the mutant strain (ION"-11 dry) was found to have the utmost rate of producing liquor after 10 days of fermenting, under the condition of original sugar concentration is 12%,acidity 3.5,sample volume 80%,inoculation volume 7% and temperature is 18~22\, the utmost rate of producing liquor is 7.69%, and 7.57% higher than that of natural strain Y02.The research methods and conclusions of this paper are as follows: First, selecting seven different dose of ion-implantation, the are 8*1014, 1*1015, 3*1015, 5*1015, 7*1015, 8*1015, l*1016(unit:Ion/cm2) respectively, with the treatment of these different doses of ion-im plantation, the curve of strain death was created and the optimal dose of ion-implantation was defined as 8*10l5Ion/cm2, under this dose of ion-implantation, the rate of strain death is 72.3%. \Second, after being treated with one time ion-implantation applied and cultivated, nine single strain colonies were selected.(10"4 dilution times). Through fermenting test, the optimal mutant strain ION' -9 of being treated was obtained. This mutant strain has higher yield producing liquor than that of natural strain Y02 for 2.67%. its utmost rate of producing liquor is 7.32% after 10 days of fermenting under the condition of original sugar concentration is 12%,acidity 3.5,samplevolume 80%,inoculation volume 7% and temperature is 18~22℃Third, after being treated with two times ion-implantation applied and cultivated, eleven single strain colonies were selected.(10-4 dilution times). Through fermenting test, the optimal mutant strain ION "-11 dry was obtained, which is No. 11 strain colony .Its shape character is shown to be dry.This mutanthas higher yield producing liquor than that of natural strain Y02 for 7.57% ,its℃ utmost rate of producing liquor is 7.69% after 10 days of fermenting under thesame conditions as that of the first time ion-implantation.Fourth, after right designation of the optimal mutant strain ION "-11 dry' s fermenting conditions, it concludes that when the beginning of the sugar concentration and acidity and the amount of inoculation and loading are 14%, 3.0, 7% and 90% respectively, and under the temperature of 18~22℃, this special mutant strain ION"-1 Idry has the extremely high yield producing liquor ability. Its utmost rate of producing liquor is 7.89% after 10 days of fermenting.Fifth, bio-characters of the special mutant strain ION "-1 Idry were studied, the conclusions are as follows: The colony character of this special mutant strain is similar to Candida, and the volume of the cell shows the ratio of length and width is bigger than 2 urn or equal to 2 urn. The physiology and biochemistry characters of this special mutant strain show that glucose, sucrose, lactose and maltose can both be fermented and assimilated and (NH4)2SO4 and KNOa are also used. The optimal growth temperature of both original strain YQI and optimal mutant strain ION"-1 Idry are 25℃.Sixth, after SDS-protein electrophoresis analysis of the optimal mutant strain ION"-11dry and the original strain Y02, the conclusion is that the optimal mutant strain ION "-11 dry has different another lane comparing to the original strain Y02.Seventh, specially to say, the optimal mutant strain ION11-11dry can also use the starch and cause the hydrolysis of it ,which is different from the original strainY02.