Studies On The Application Of Dye Dimer In The Determination Of Biology Macromolecules As Fluorescence Probe

Protein and DNA are important biology macromolecules. The quantitative determination of protein and DNA is a common analysis contents in biology chemistry and life science. So it is very important to find fluorescence probe with high sensitivity and low detection limit. In this thesis the in situ brilliant cresyl blue (BCB) dimer with weak fluorescence in the solution of anionic surfactant (AS) sodium lauryl sulfonate (SLS) was used as fluorescence probe. At the same time, thenew method for the determination of bovine serum albumin(BSA) was built. Thelinear range was 0-7mg/L and the detection limit(3 o /k)was 3.66 10-3 mg/L,theR.S.D for the determination of 2.00mg/L BSA was 2.77%. So the method was accurate and reliable.The in situ nile blue (NB) dimer with weak fluorescence in the solution of anionic surfactant sodium dodecylbenzene sulfonate-6(DBSS) was also used as the fluorescence probe for the determination of total proteins in human serum. The determination results achieved by this method were in good agreement with clinical data provided by the hospital when this method was used to determinate total proteins in real human serum samples.Dye dimer is effective form of energy transfer, too. In this thesis, the energy transfer system of acridine orange(AO)-rhodamine B(RB) dimer was built for the determination of DNA as fluorescence probe. Under the experimental conditions, the linear tange of this assay was 0.33~1.33mg/L for fish sperm DNA and 0.33-3.33mg/L for calf thymus DNA,the detection limit was 1.63xlO"3mg/L for fish sperm DNA and 1.52x10-3 mg/L for calf thymus DNA,respectiveIy.The R.S.D for the determination of 1.00mg/L DNA was 2.40% for fsDNA and 2.00% for ctDNA,respectively.