Molecular Test Of Variant Wheat After Injected Pea DNA By Pollen Tube Pathway

Under the conditions of high wheat yield in china, it is a insistent task for breeders to improve wheat quality. In the experiment pea genomic DNA were intrAuced into wheat Lu22 through the pollen tube pathway in order to enhance wheat protein content and improve wheat quality. Then the variant wheat T220X were studied from gliadin, isoperoxidase, the difference in DNA and protein content. The main results were as follows.1. The results of seed gliadin electrophoresis showed that there were difference in zymotype between Lu22 and T220X, which verified that the injection of exogenous DNA influenced the component of gliadin. It was affirmed preliminarily that exogenous DNA had made an effect on the wheat 1 Chr. or6Chr.2. The peroxidase activity of variant T220X was obviously higher than acceptor Lu22 at seedling stage. The result of peroxidase electrophoresis indicated that there were difference both in the depth and in the amount of zymogram between Lu22 and T220X.. So it was concluded that the variety of peroxidase was the result of change of heriditary substance.3. The result of RAPD analysis showed there were many differences in DNA between variant T220X and acceptor Lu22. After the amplication of primer S103, there was a specific fragment about 850bp in T2201; after the amplication of primer S74, there were two new fragment about ISOObp and 400bp in variant T2202. The amplified prAuct of SI 18 had a 480bp new fragment in variant T2204. In variant T2203 there were two specific fragmentsabout 980bp and 550bp after ampliation of primer S64. It is possible that exogenous DNA fragment integerated into acceptor genome, changing gene base sequence or base deletion or base insertion, inducing to mutant at gene level.4. The detecting of protein content indicated that there was a obviously enhandcement of protein content in variant T220X than in acceptor Lu22. The result affirmed that it is available to improve protein content through pollen tube pathway.These results indicated that it is practicable inducing exogenous DNA into wheat through pollen tube pathway to obtain taget plant. There are great potential power to improve wheat quality by this way.