Characteristics, Examination Of Mutants And Analysis Of 16S RDNA Sequences Of Phenol-Degrading Phen8 Strain

PhenS, an isolate of phenol degradation, was able to utilize 85% substrate in 1-lOmM phenol medium in 24 hours. Cell metabolism were enhanced by the increasing concentration within 0-7mM. The degradation was not obvious until log phase, which was delayed by higher concentration. LB and A15 mediums were observed to be helpful to the degradation. 2% was suggested the optimal amount of inoculation The strain was resistant to Sm, utilized a broad of carbon source and grew well from 15 to 37"C and pH 5 to 10.Phenol hydroxylase gene was demonstrated to be located in the chromosome of phenS, and the yellow product was visualized in the course of degradation, which indicated that the oxygenation of phenol through meta-pathway. Cell was observed non-induction by catechol. Glucose was demonstrated to retard the degradation. Furthermore, it was verified that xylR gene encoded by pTOL, to some extent, repressed the degradation.This strain was demonstrated to homogenous to Pseudomonas stutzeri according to the alignments of 16S-rDNA sequences between the phenS and the known strains (Registration No ofphenS is AF284764 in GenBank).Four mutants with Tn5 integrated into the chromosome of phenS, losing the function of degradation, are some kinds of basic material for the future study on the gene location.