Salt Tolerance Conferred By Overexpression Of A Suaeda Salsa Glutathione-s-Transferase Gene In Arabidopsis

High salt stress disrupts homeostasis in water potential and ion distribution. This distribution of homeostasis occurs at both the cellular and the whole plant levels drastic changes in ion and water homeostasis lead to molecular damage, growth arrest and even death. To achieve salt tolerance, three interconnected aspects of plant activities are important. First, damage must be prevented or alleviated. Second, homeostatic conditions must be re-established in the new, stressful environment. Third, growth must resume, albeit at a reduced rate.The nature of the damage that high salt concentrations inflict on plants is not entirely clear. An important cause of damage might be reactive oxygen species (ROS) generated by salt stress. To protect themselves against these toxic oxygen intermediates, plants employ defense systems that include the enzymes such as superoxide dismutases, catalases, ascorbate peroxidase,glutathione-s-transferase(GST) and glutathione peroxidase(GPX) that catalyze the scavenging of ROS.Some results indicate that GST could be a critical factor in protecting plants from stress-induced oxidative damage.It is very important for us to study the function of gene through studying where and when it can express. At present, many advanced technology have been invited which can deal with gene expression analysis in large scale, for example, overexpressing one specific gene in transgenic plant can be used to study the function of this gene.Construct the GST cloned from Suaeda salsa cDNA library into prok2. Then, an integrated vector of GST and NPTII of prokll plasmid was constructed and transformed into Arabidopsis by Agrobacterium tumefaciens. Transformants were screened by their ability of growing on media containing kanamycin. The transgenic plants were confirmed by PCR and PCR-southern and the overexpression of this transferred gene was confirmed by RT-PCR and Northernanalysis. At last, transgenic Arabidopsis and wild type Arabidopsis were planted on MS media owning 100 mM NaCl. Though there is difference between them, the difference is not so clear, planting them in soil and washing them with Hoagland including 100mM NaCl, I find the transgenic Arabidopsis is a little higher than the wild type. So overexpression of Suaeda salsa GST in transgenic Arabidopsis has some difference to its salt tolerance.