Cloning, Fuctional Analysis Of HMG Proteins From Pharbitisnil, Maize And Cucumber

The high-mobility-group proteins (HMG) belong to a highly abundant class of nonhistone chromosomal proteins found in eukaryotic organisms. HMGHbox is a common conserved region of HMG protein and playing a important role in cell. HMG proteins divides three familys:HMG-1/2 family, HMG-I/Y family, HMG-14/17 family. And HMG proteins associated with actively chromatin. HMG protein may be involved in various cell functions, including cellular differentiation, sex differentiation, nucleosome transcription and replication. It was reported members of HMG-box containing protein were characterized from animals, such as hUBF and SRY. But only a few HMG protein were characterized from plants.It was reported an cDNA encoding HMG protein was cloned from Pharbitis nil(HMGl). In order to indentify more HMG-box containing protein in Pharbits nil, we cloned another cDNA -HMG2 by using methods like RT-PCR, which encoding HMG-box containing protein and high mobility with HMGl, we studied its function by using methods like gene clone, transgene teconology, Southern and Northern blot hybridization analysis methods etc. The gene expressing of PNIL HMGl is obviously regulated by a circadian rhythm which is thought to be involved in flowering and affected by night break treatment which inhibit flowering, but there no effection on HMG2 DNA abundance. HMG2 may play a housekeeping role in the general regulation of gene expression in Pharibitis nil. At same time we cloned HMG-box from Maize, Cucumber, and HMG-box of Cucumber was first reported.