Studies On The Effects Of Jerusalem Artichoke On The Growth Of Bifidobacteria

In order to provide theoritical foundation for the utilization of Jerusalem artichoke(JA) resource,culturation of Bifidobacteria and development of Bifidobacteria products.this paper rather systernly studied the effects of JA on the growth of Bifidobacteria,using Jerusalem artichoke juice(JAJ) and Jerusalem artichoke powder(JAP) as experimental material .In the first place, the effects of JA on the growth of Bifidobacteria in vitro were studied.The results indicated that: JAJ could stimulated the growth of B.longum(Blm) and B.bifidwn(Bbm);The more JAJ content was added,the more promoting action was apparent; the promoting action also varied from the different species.Adding JAP(3%) to skim milk could decrease the time of milk-solidfying of Blm and Bbm,and could increase the acid production in skim milk of the strains tested.Secondly, Bifidobacterium medium was developed using JAJ as main material. The results showed that: Adding Tryptone,soy peptone.beef extract,com extract and Cys-HCl to JAJ could obviously promote the growth of Blm and Bbm;By the orthogonal experiment of three elements and three levels,a satisfying JAJ compound medium was acquired which included corn extract(0.3%), soy peptone(0.05%)and Cys-HCl(0.025%).Nextly,after establishing a selective Bifidobacterium medium, the effects of JAJ on the growth of Bifidobacteria in vivo were studied,using healthy mouse of Kunming species as experimental animal. The results indicated that:JAJ could selectively stimulate the reprduction of Bifidobacteria in vivo and inhibit the growth of E.coli which is a main parasitic basterium in human intestinal tract;Moreover,JAJ could apprarently improve intestinal tract function.In tested group,the mice excreted smoothly and the faecal particles of mice were big and wet,but in control group, the faecal particles of mice were small and dry.lt was suggested that inulin may be the important effective component in JAJ which promoted the reprduction of Bifidobacteria in vivo .At last, the effects of JA on the bile salt resistance of Bifidobacteria were studied.The test proved that:Deoxycholic acid Na-Salt(DCA-Na) had intensely toxical action on Blm and Bbm;Adding glucose and fructose in media could decrease the lexical action on Bbm.but inulinand JAP had not apparent effect.