Cloning And Sequence Analysis Of TM-1 Gene Of Mycoplasma Gallisepticum Strain H₃

The pathogen of Chronic Respiratory Disease(CRD) is Mycoplasma Gallisepticum(MG). CRD can lead to heavy economic losses. Living vaccine and killed vaccine have some formidable drawback, so biological technology vaccine , for example, gene vaccine and genetic engineering vaccine, is under the circumstances. Abtaining immunogen is the key to biological technology vaccine, and in this report , cloning and sequence analysis of TM-1 gene of MG strain HS was done . A set of oligonucleotid primers was designed according to the published sequences of MG strain 85. A fragment of 0.8Kb was amplified by PCR from DNA of strain HS. and cloned into plasmid veator PUCm-T and identified by sequence analysis. By comparing the cloned TM-1 DNA fragment sequence with corresponding published Se sequence. It was found that H3 TM-1 gene have 96.57% and 95.42% homologies with S6 TM-1 gene in nucleotide and amine acid level . The result of this experiment provide an important basis for further study on gene vaccine and genetic engineering vaccine of strain Ha.