STUDY OF CHANGES OF FSH,LH,17β-E₂,P₄ CONCENTRATIONS IN PLASMAS OF FEMALE OSTRICHES (STRUTHIO CAMELUS) BY RIA AND IEMA UNDER DIFFERENT PHYSIOLOGIC CONDITIONS

During ostriches (Struthio Camelus) reproduction, the blood samples were collected in 12 female ostriches at 4-hour intervals during 72 hours. The concentrations of FSH,LH ,17β-E2 and P4 in these ostriches plasmas were determined by RIA and IEMA. Changes of FSH,LH ,17β-E2 and P4 concentrations in plasmas were probed in different physiologic conditions. Usage of RIA and IEMA were compared and laying activities of these ostriches were monitored by these 4 hormones profiles in plasmas. The results of FSH,LH ,17β-E2 and P4 concentrations in plasmas were summarized as follows: To the continuous-laying ostriches , the highest FSH concentration was 5.33±0.67mIU/ml(RIA) or 4.87±0.46mIU/ml(IEMA). the highest FSH concentration was 5.33±0.67mIU/ml(RIA) or 4.87±0.46mIU/ml(IEMA). the highest LH concentration was 5.04±0.45mIU/ml(RIA) or 17.19±0.97mIU/ml(IEMA), and it appeared 12~2h before laying. the highest 17β-E2 concentration was 0.932±0.049ng/ml(RIA) or 0.3448±0.069ng/ml(IEMA), and it appeared 10~2h before laying. the highest P4 concentration(8.82±0.49ng/ml(RIA) or 7.105±0.549ng/ml(IEMA) appeared 18~10h before laying, and its concentration changed slowly. To not-continuous laying ostriches , before they lain the latest egg, the highest concentrations of FSH,LH ,17β-E2 and P4 in plasmas (RIA) were not determined respectively. But before they lain the second latest egg, the top concentrations of these 4 hormones (RIA) were similar with the continuous-laying ostriches' 4 hormones concentrations respectively (p>0.05).To no-laying ostriches, the highest concentrations of FSH,LH and 17β-E2 in plasmas (RIA) were not appearant respectively. But their basical values were the same as not-continuous laying ostriches' and continuous laying ostriches' except of P4 (p>0.05) Difference of the highest concentrations of FSH,LH ,17β-E2 and P4 were significant between by RIA and by IEMA (p<0.05). The correlative coefficient of the concentrations of FSH,LH ,17β-E2 and P4 (RIA) of ostriches in plasmas in RIA and IEMA were 0.932, 0.9151, 0.91832, 0.8305 respectively. These two results reflected change of FSH,LH ,17β-E2,P4 between ostriches lay two eggs when both methods used. RIA and IEMA have practicable value for assaying FSH,LH ,17β-E2 and P4 concentrations of the ostriches in plasmas.