The Studies On Expression Of 1197 Gene Of Prawn Baculovirus And On Purifications Of The Expressed Product

since 1993, death of many breeding prawns appeared in Chinese coastal provinces. This kind of phenomena led to great economic loss, and many experts from home and abroad attached importance to it. After a long period research, the cause of this epidemic disease is a kind of non-inclusion baculovirus, and some research result concerning this baculovirus ~ histological and pathological character have reported. In previous report we have cloned a putative early and late transcriptional gene 1197 of prawn baculovirus. we have designed two ribozymes in order to cut the gene in vivo and inhibit baculovirus from prolitbration, as well as control the disease. In order to detect the biological function of this gene, we have inserted 1197 gene into expressing vector pGEX-3X. The fusion protein expressed with high yield was obtained, but it was found that the fusion protein locates in inclusion bodies of E. coli and it causes trouble in its purification. We found that the expressed product with MW. of 66kD could be purified from inclusion bodies using a serious of treatments of inclusion bodies with urea *The First Military Medical University and guanidine hydrochloride and then by sequential FPLC chromatography. We can research the function of the protein during the baculovirus genes transcription and translation. The protein denature was caused by urea will or will not affect the function of the protein still need more confirmation.