Studies On Production And Regulation Of The Inulinase By Aspergillus Niger Ur-2

The process of inulinase yielding from Aspergillus niger Uy-2 was studied . The rate of inulinase yielding and that of pH declined were fast in the initial fermentation phrase.The production of extracellular inullnase is less than other time, when pH is between 4.38?.22. The maximum of extracellular inulinase acticity(EIA) and the minimum pH showed at the same time(192 h). There were two soluble protein(SP) peaks occurred in the fermentation course. The fist one was given at 72 h, which was compose of the impure protein,and the other showed the synchronism and the increase of EIA. Analysis of the changes of biomass(BM)showed that the optimal extracellular inulinase yielding phase was the period of 120?92 h.(anaphase of the logarithmic growth phase and the whole stationary phase). The forming and the dynamic changing of inulinases, which were extracted from supernant cell-wall and introcell, were studied by polyacrylmidegel electrophoresis(PAGE). Three sections of inulinase presented significant difference, and there were no extracellular inulinase compositions in cell. The secretory mode of extracellular inulinase was associated with translation. The maximum of EIA was showed at 192 h, inulinase synthesis accompanied the cell growth, the enzyme was continued a period time when the groth of cell was stationary phase. The intracellular inulinase activity(llA) and the cell-wall inulinase activity(CIA) were decreased quickly at 24?8 h, the llA decreased and the CIA hardly varied after 48 h. Evironmental agent effected inulinase synthesis and secretion were studied. lnulin was one inducer to the synthesis of extracellular inulinase. The degradation production of glucose and fructose had no inhibition on extracellular inulinase synthesis. The EIA was raised 20.9W by adding glucose during fermentation period. The yielding of inulinase wasn't effected by Tween 80, The EIA was decreased by the forming of mycellum pellet destructed by adding the glass ball, wheras the EIA was increased 30.72U by adding lywallzyme(120 h).