Detection Of Expression Of Basic Fibroblast Growth Factor(bFGF),Fibroblast Growth Factor Receptor-1(FGFR-1) And BFGF MRNA In HEP-2 Laryngo-tumor And 803 Gastro-tumor Cell Lines

Basic fibroblast growth factor (bFGF) promotes cell growth and angiogenisis. Many reports show that it plays an important role in tumor formation and development. In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I (FGFR-I) and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.The results show that in these two cell lines cultured in vitro, the bFGF positive staining exists in the whole cell, and the staining intensity of nucleus is higher than that of cytoplasm. The staining of FGFR-l and bFGFmRNA mainly locates in nucleus.In the solid tumor tissue formed by HEP-2 laryngo-tumor cell ,bFGF,FGFR-I and bFGFmRNA positively stained cells are those tumor cells surrounding the necrosised zone, and the staining of nucleus is stronger than that of cytoplasm. The capsular tissue of tumor also shows some positive staining to a light extent.In the solid tumor tissue formed by 803 gastro-tumor cells, bFGF and FGFR-l positive staining locates in tumor cells and capsular tissue, the staining of capsular tissue is striking. bFGFmRNA positive staining mainly exists in tumor cells, and only a few cells of capsular tissue are stained positively.In conclusion, the two cell lines express bFGF, FGFR-l and bFGFmRNA either in vitro or in vivo, after conjugation of bFGF with its high affinity receptorGFR- I, bFGF resides in nucleus wherein excises its biological function. bFGF produced by tumor capsular tissue may also take part in the formation and development of tumor tissue.