Several Simple Methods Of Research Photosynthesis Process

Photosynthesis is the most biological material and energy sources in the earth, the vital link between biological and earth, the widespread and unique plant physiology process, and the most important biological production restricted ecological system in physiological processes. Researching photosynthesis has great significance on production practice and theory. Therefore, study the energy conversion, electron transfer, energy transmission of photosynthesis process has the vital significance for understanding and mastering many important natural development regularity and artificially technology. Photosynthesis light energy efficiently absorpt, transfer and convert the energy and the microscopic mechanism its controled is the core issue of basic research in the current international photosynthesis. The electronic transfer is the basic process in life activity, most of the life activities such as organism of hemoglobin, the process of carrying oxygen chloroplast in the mitochondria, photosynthesis and respiration, are all closely related with the electronic transfer process, and most life process was happening on biological membrane. The interface including liquid/liquid interface and solid/liquid interface are thought to be more simple modeling in biological membrane model. Based on this, the paper mainly used porphyrin compounds, chlorophyll and benzene quinone as the matching material, and systematically study the electron transfer process and photoelectric behavior on the modeling of biological membrane interface (liquid/liquid and solid/liquid). This has certain significance for photosynthesis process studies.Along with the rapid development of science and technology and interdisciplinary mutual infiltration, interface chemical study will also meet more and more challenges and opportunities, and more precision technologys and reasonable methods for the application of recoganizing nature, will have a broad prospect.Combining with Scanning Microscope Scanning Microscope (Electrochemical Electrochemical, SECM) technology, thin layer cyclic voltammetry and SECM - uv-vis illuminant coupled research the photoelectric conversion process in photosynthesis process and the electronic transfer process of metallic porphyrin in bionic interface. This paper is divided to four parts, mainly include the following content:1. Summarizes the development and research significance of photosynthesis, simple told the photosynthesis process research methods which commonly used. Review the development background, basic configuration and application prospect of liquid/liquid interface electrochemical. Told the details of two main kinds research methods: SECM and thin-layer cyclic voltammetry (TLCV) work principle, quantitative analysis theory and application, and summarizes the situation of photoelectric chemistry.2. Discussing the primal reactions of photosynthesis used iron porphyrins and benzene quinone as redox in the paper, using different driving force come from different substituents simulate the different light intensity, and discussed relations between driving force and electronic transfer reaction. We found that the reaction rate is increases with increasing of driving force when the driving force is smaller ,but decreases with increasing of driving force when driving forces is larger. This results indirect may process of plant photosynthesis, not the light intensity more light the electric conversion efficiency is higher, during which there may be an optimal illumination, when light intensity reach this dot, photoelectric conversion efficiency achieve to maximum. In addition, use SECM also studied the electronic transfer process between iron porphyrins in nitrobenzene and benzoquinone in water, and the influence the concentration of reaction matter and common ion to the rate constant are discussed.3. For the advantage of TLCV method in liquid/liquid interface, we used it to simulationed the photosynthesis original reaction process simply with benzene in aqueous phase and iron porphyrins in organic phase and study the electron transfer process. Then comparaed it with SECM and found that the experimental results come from this two methods are coincide. Which not only confirmed the reliability of TLCV but also expand the study scope of TLCV. 4. Application SECM and ultraviolet-visible illuminant coupled to study the chlorophyll'photoelectric conversion process which extracted from spinach, using I - t technology optimized the conditions of reaction process, studied the electronic transfer between chlorophyll and benzene system, and the influence of vapor concentration in water to the reaction rates.