Modeling For Genome-scale Metabolic Network Of Zymomonas Mobilis Subsp. Mobilis ZM4

Reconstructing a genome-scale metabolic model means great theoretical and practical significance by facilitating both the biological theory discovery and research and metabolic engineering for strain improvement.Zymomonas mobilis is a famous ethanologenic bacterium for it could produce ethanol at a yield close to the theoretical one on glucose. Zymomonas mobilis is also a model orgnism for studying ED pathway as well as pyruvate decarboxylase and other enzymes. So genome-scale metabolic modeling for Zymomonas mobilis is important no matter which side we take.The Zymomonas mobilis ZM4 model reconstructed in this paper is the first genome-scale model for Zymomonas mobilis in the world.Firstly, a general process for genome-scale metabolic modeling and a brief principle for simulation was introduced in the paper. Secondly, the process to reconstruct the genome-scale metabolic model for Zymomonas mobilis was elaborated in detail, which mainly includes: downloading and extracting primary data from KEGG and other database, validation of the primary data, construction of the reactions database, refinement and validation of the model.Finally, more complex simulations were imposed on the model, such as robustness analysis, PhPPs analysis and gene deletion, and the results of the different simulation were not only in agreement with each other but also accord with experiment data. The relation of the stability of ethanol production and growth rate was discovered through a simulation. A innovative PhPPs analysis was made on varying inhibition of ethanol production and growth; and another innovation is simulating batch fermentation by introducing diffusion mechanism of the glucose uptake to the model.