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Fungi Community Structure And Physical And Chemical Factors Correlation Analysis: Hailang River Downstream

Posted on:2012-03-25Degree:MasterType:Thesis
Country:ChinaCandidate:D Y LvFull Text:PDF
GTID:2120330335973203Subject:Aquatic biology
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This thesis experiments with Hejiacun section in Hailang area of Mudanjiang River, collected samples from ten typical sampling stations in March, May, July, September, November 2009 and January 2010, by means of molecular biology research to study the diversity of fungi which grow in this area. Fungi community structure and biodiversity were examined with multivariate and analysis of the correlated water environmental physical and chemical factors, discussed based on results of SPSS Statistics 17.0, Pearson analysis, and Shannon-Wiener index. This paper aimed to provide a guideline of environmental administration and protection, and restoration of the ecosystem in Hailang River of Mudanjiang.From samples collected in Hejiacun section of Hailang River area,32 species of fungi were isolated, belonging to 5 Order,7 Family,13 Genus, among which 24 species were identified to species rate,8 species were identified to genus rate.15 species belonged to Aspergillus,6 species belonged to Penicillium,2 species belonged to Trichoderma, Rhizopus, Saccharomyces, Phanerochaete, Piricaulalia, Alternaria Nees, Microsporum gruby, Geotrichum Link, Cercospora Fres, Oospora and Leptomitus contented 1 species each.Total of dominant fungi was 16, including Aspergillus nig, Aspergillusjanus, Aspergillus fumigatus var fumigates, Rhizopus sp., Trichoderma harzianum, Microsporum gruby sp., Saccharomyces cerevisiae, Aspergillus niger, Aspergillus flavus Link:Fr. var. flavus, Aspergillus restrictus, Penicillium sp., Penicillium thomii, Aspergillus carbonarius, Penicillium janczewskii, Aspergillus tubingensis, Leptomitus agardh.The results showed that fungi communities in Hailang River fluctuated with season significantly. Total of species isolated in March,November 2009 represent winter was 33 and 31, which were the most among all the year; the least of the whole year was 21, which was isolated in July 2009 represented summer. Fungi biomass distributed the most in May 2009, the least in January 2010; spatial distribution showed fungi biomass in sampling station 1,7,8,9, 10 higher than in 5,4,3.The whole year diversity index showed that Shanno-Wiener index was from 0.00 to 2.20, the index reached 1.79 in Jan.2010 which was the highest of the whole year, while the lowest was 0.72 in Sep.2009. From the spatial distribution of Shanno-Wiener index, sampling station 5,9 highest and 2 lowest.Correlation analysis showed fungi diversity significant related with water temperature,P content; Shanno-Wiener index of fungi significant related with P diversification, unrelated with N diversification. The result of relational analysis of fungi biomass with environmental factors showed total biomass unrelated with water temperature, speed, Fe3+, N, P, COD.
Keywords/Search Tags:Hailang River, fungi community structure, physical and chemical factors, correlation analysis
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