Molecular Screening And Functional Analysis Of Specific Genes In The Hydrothermal Vent Crab, Gandalfus Yunohana

MRNA transcriptional profiles between hepantopancreas of the hydrothermal vent crab, Gandalfus yunohana, and its close-related non-vent counterpart, Portunus trituberculatus, was compared by the method of DNSH [DSN (duplex-specific nuclease)-mediated normalization and subtractive hybridization] in our research to explore biological survival mechanism of vent animals. Through constructing the full-length normalized subtractive cDNA library, we identified 51 different transcripts which have similarity to known genes in GenBank. We selected thioredoxin from these genes to construct prokaryotic expression system. After identifying and analysising the complete open reading frame of thioredoxin, we detected the activity of its expression product. Meanwhile, we obtained the complete cDNA sequence of heat shock protein 90 in G. yunohana through the method of rapid amplification of cDNA ends. After analysis of its sequence, we construct the prokaryotic expression system and detected its cell protection function under heat stress in prokaryotic cells. Our research provides the first genetic information on thioredoxin and heat shock protein 90 in hydrothermal vent species and our data lay the foundation for future research of biological resilience and survival mechanism in the special environment of hydrothermal vents.