Synthesis And Properties Of Ratiometric Fluorescent Probes And Mesoporous Nanoparticles For Hypoxia Sensing

Hypoxia is the essential character of human solid tumors. Hypoxic cancers are resistant to traditional radiotherapy, phototherapy, and chemotherapy, and also to conventional cancer immunotherapy. In this paper, two kinds of hypoxic molecule probes based on 1-Methyl-2-nitroimidazol-5-ylmethyl carbamates were designed and synthesized. The results show that the probe with the fluorophore of 6-amino coumarin has no significant changes in absence and presence of nitroreductase, but the hypoxic-oxic fluorescence differential incubated with this probe could change slightly between oxic and hypoxic cells separately. The probe with the fluorophore of 4-amino-1,8-anhydride naphthalene has significant ratiometric changes in absence and presence of nitroreductase, and the hypoxic-oxic fluorescence differential obviously between oxic and hypoxic cells incubated with this probe separately.Recent breakthroughs in the application of mesoporous silica nanoparticles, such as adsorption, catalysis, sensing, and separation. In this paper mesoporous silica nanoparticle and magnetic mesoporous silica nanoparticle were designed and synthesized via the monomer of 2,7-nitro-[1,2,5]-benzoxiadiazol hypoxia probe and 9-nitro-acenaphtho[1,2-b]quinoxaline hypoxic probe. The results show that they are the representative mesoporous structure, and the hypoxic-oxic fluorescence differential obviously between oxic and hypoxic cells incubated with these nanoparticles separately.