Preparation Of Nanozyme-Based SERS Probe And Its Detection Application

The accurate detection of markers such as cationic dyes and glucose is an important means for environmental monitoring,food safety supervision and medical diagnosis.However,in practical applications,most markers exist in complex matrix containing various interfering impurities,which makes traditional detection methods generally have problems such as low sensitivity,cumbersome steps or high cost.It is of great significance to develop an anti-interference,simple,convenient and low-cost detection method.In this paper,based on functional metal-organic framework（MOF）nanozyme materials,a series of highly specific,low-cost and recyclable surface-enhanced Raman scattering（SERS）probes are designed and fabricated.The application of such probes is explored and expanded.It has been successfully used in the detection and analysis of important markers such as cationic dyes and glucose in complex samples,providing a reliable analytical tool for the accurate detection of markers in complex samples.The main innovative research results include:Ni@MIL-100（Fe）@Ag nanowires（NMAs）,a recyclable SERS probe for detecting cationic dyes in pond water is designed and fabricated.The probe is built on magnetic thorn-like nickel nanowires with high specific surface area,which can efficiently support MIL-100（Fe）and facilitate magnetic collection.Functional MIL-100（Fe）not only effectively improves the modification efficiency and robustness of Ag on the surface of the probe,but its enrichment performance and size screening performance can also enable the probe to specifically adsorb the target.So,NMAs can effectively eliminate the interference of impurities and sensitively detect cationic dyes in complex pond water with a limit of detection as low as 10^-10M.In addition,by making full use of the peroxidase-like catalytic activity of MIL-100（Fe）,the NMAs can quickly realize catalytic self-cleaning and reusability at least 10 cycles,which effectively reduces the detection cost and provides an economical detection solution for cationic dyes in complex waters.A highly sensitive glucose detection method suitable for complex matrix is proposed.In this method,MBs@MIL-100（Fe）@Ag（MMA）with excellent SERS activity and peroxidase-like catalytic activity is fabricated as SERS probe.TMB,which can be specifically enriched and detected by MMA,is used as the reporter.Highly sensitive detection of glucose is achieved by the catalytic cascade reaction of MMA and glucose oxidase.The linear detection of this method ranges from 20μM to 1000μM.Moreover,the functional MMA has excellent anti-interference properties and detection sensitivity in various complex matrix.So,this method can accurately detect the glucose in saliva and colored beverages.In addition,the excellent catalytic self-cleaning performance of MMA enables the reuse of probes at least 3 cycles,reducing the detection cost of this method.This method provides a new strategy for the precise detection of glucose in complex matrix.A microfluidic SERS detection chip,MBs@MIL-100（Fe）@Ag chip（MMA chip）,is constructed,which can be used for multiplex detection of cationic dyes in pond water.The chip is prepared by 3D printing technology and has a multi-channel microfluidic structure.Compared with the traditional SERS probe,the MMA chip can run automatically by microfluidics and integrate the preparation and detection steps of the functional SERS probe MMA.The MMA chip has the advantages of high automation,small volume,low reagent consumption and high detection efficiency.So,it can be used for monitoring the catalytic reaction process in real time.In addition,based on the excellent detection specificity of MMA in the multi-channel chip,the MMA chip can also be used for multiplex detection of cationic dyes in pond water,providing a new strategy for the efficient preparation and detection application of functional SERS probes.