Research On Drug Law Of Professor Qi Yuan-fu In Treatment Of Breast Cancer And Experimental Study On The Regulation Of Wnt/?-catenin Signaling Pathway By Core Recipe

Objective:1.To study drug law of Professor Qi Yuanfu in treatment of breast cancer,and extract the core recipe and new recipe of Professor Qi Yuanfu in the treatment of breast cancer.2.To study the effect of breast cancer core recipe on the growth,proliferation,invasion and Wnt/?-catenin signaling pathway of human breast cancer cells MDA-MB-231.Methods:1.Collected professor Qi Yuanfu 's recipe for treating breast cancer,used Traditional Chinese Medicine Inheritance Support System for data analysis,obtained the results of drug frequency,recipe rule,new recipe analysis and so on,and extracted professor Qi Yuanfu's core recipe for treating breast cancer,and discussed its characteristics of treating breast cancer.2.Prepared serum containing core recipe of breast cancer and in vitro cell culture experiments were conducted.MTT assay and cell count method were used to observe the effect of serum containing core recipe of breast cancer on the growth and proliferation of human breast cancer cells MDA-MB-231.HE staining was used to observe the effect of serum containing core recipe of breast cancer on the morphology of human breast cancer cells MDA-MB-231.The influence of serum containing core recipe on the migration and invasion ability of human breast cancer cells MDA-MB-231 was observed by scratch test and transwell experiment.Western blot experiment was conducted to observe the effect of the core recipe of breast cancer on expression levels of the proteins of ?-catenin,Cyclin D1 and c-Myc in Wnt/?-catenin signaling pathway.Results:1.The four characters of the drugs were mainly cold,warm and flat?The five tastes of the drugs were mainly bitter,sweet and spicy.The meridians were mainly hepatic meridians.Frequency of the top ten drugs were radix bupleuri,fleas,glycyrrhiza,finger citron,cortex albiziae,coix seed,thunberg fritillary bulb,radix pseudostellariae,oldenlandia,atractylodes.Analysed common drug pairs based on association rules: radix bupleuri,fleas;finger citron,cortex albiziae;atractylodes,fructus aurantii;solanum lyratum,duchesnea indica.Got 7 new recipes for breast cancer from professor Qi Yuanfu.2.Core recipes of breast cancer:radix bupleuri 15 g,fleas 15 g,glycyrrhiza 15 g,finger citron 30 g,cortex albiziae 30 g,coix seed 45 g,thunberg fritillary bulb 30 g,radix pseudostellariae 30 g,oldenlandia 24 g,atractylodes 15 g,selfheal 15 g,zedoary 15 g,fructus aurantii 15 g,solanum lyratum 15 g,duchesnea indica 15 g.3.All drug-containing serum groups in MTT experiment showed inhibitory effect by drug intervention after 24 h.With the increase of drug concentration,the inhibitory effect gradually increased.After HE staining,the number of cells in each drug group decreased significantly,cells became smaller,rounded and polygonal,cells were clustered together,and there was no obvious difference between cytoplasm and nucleus.The serum containing core recipe of breast cancer can inhibit the growth,proliferation,migration and invasion of human breast cancer cells MDA-MB-231.The combination of adriamycin has a synergistic effect.The expression of ?-catenin,Cyclin D1 and c-Myc can be down-regulated in the serum containing the core recipe of breast cancer and then correct the Wnt/? catenin signaling pathway disorders.Conclusion:1.Based on the traditional Chinese Medicine Inheritance Support System,conduct data analysison to find professor Qi Yuanfu's treatment of breast cancer,research professor Qi Yuanfu's drug law and provide more options for clinical treatment of breast cancer.2.The core recipe of breast cancer has the effect of inhibiting the growth,proliferation and invasion of breast cancer.The mechanism may be related to the regulation of Wnt/?-catenin signaling pathway,and further inhibit the metastasis of breast cancer.