Inflammation-induced JMJD2D Promotes Colitis Recovery And Colon Tumorigenesis By Activating Hedgehog Signaling

Histone demethylase JMJD2D can promote gene expression by specifically demethylating H3K9me2/3.The role of JMJD2D in colitis and colitis-associated colorectal cancer(CRC)progression remains unclear.Here,we showed that colonic JMJD2D was induced by TNFα during dextran sulfate sodium-induced colitis.JMJD2D-deficient mice exhibited more severe colon damage and defective colon regeneration due to impaired Hedgehog signaling activation after colitis.JMJD2D knockdown in CRC cells suppressed Hedgehog signaling,resulting in reduced CRC growth and metastasis.Mechanistically,JMJD2D promotes Hedgehog target gene expression through interacting with Gli2 to reduce H3K9me3 levels at the promoter.Clinically,JMJD2D expression is overexpressed and positively correlated with Gli2 expression in human inflammatory bowel disease specimens and CRC specimens.The JMJD2D inhibitor 5-c-8HQ or aspirin synergizes with Hedgehog inhibitor vismodegib to inhibit CRC cell proliferation and tumorigenesis.Collectively,our findings unveil an essential role of JMJD2D in activating the processes of colonic protection,regeneration,and tumorigenesis.