Study On Reducing Toxicity And Enhancing Efficacy Of Tripterygium Wilfordii In Combination With Licorice

Purpose:Based on Chinese compatibity theory, the model, represented by toxic Chinese medicine Tripterygium, reveals the scientific content of traditional Chinese medicine theory on toxicity reducing and efficacy enhancing, and initially reveals the effective material basis of toxicity reducing and efficacy enhancing about toxic medicine Tripterygium compatible with detoxifying herbs Licorice.Material and method:1. Establish a method for the determination of Tripterygium and Licorice, under the research of the extraction of the two medicine via high performance liquid chromatography.2. Initially explorer the the material basis of toxicity reducing and efficacy enhancing of Tripterygium compatible with Licorice, by determining content of the composition in the medicines.3. Comparing the pharmacokinetic parameters of triptolide compatibility with glycyrrhizic acid, investigate the mechanism of toxicity reducing and efficacy enhancing. 4. Evaluate the effect of toxicity reducing and efficacy enhancing on Tripterygium compatible with Licorice by studies on toxicological and pharmacodynamic of the two medicines.Results:1. The extraction process of tripterygium and licorice studied by high performance liquid chromatography proved that the process is stable, reliable, in order to further study the two drugs provide an experimental basis.2. After tripterygium compatible with Licorice, the content of triptolide decreased, the effect may relate with the complexation of ammonium and licorice;3. On the pharmacokinetics of two drugs, the study found that with compatibile of licorice ammonium triptolide reduced the maximum plasma concentration and prolonged half-life;4. The toxicology and pharmacodynamic results of tripterygium and Licorice show that licorice has good effect of toxicity reducing and efficacy enhancing. Conclusion:1. Investigating the content of triptolide in the Tripterygium, a antirheumatic Chinese medicine, compatible with Licorice, the result showed that glycyrrhizin can reduce the content of triptolide, and supported the theory of toxicity reducing and efficacy enhancing.2. Reseaching the pharmacokinetics of troptolide after compatible with Licorice, the results showed that the half-life (T_1/2) of triptolide extended and the maximum plasma concentration (Cmax) of triptolide decreased. Under the influence of licorice, anti-rheumatic of triptolide is mild and toxicity decreased, effect become longer.3. The studies showed that the anti-rheumatic effect of Tripterygium increases, via anti- inflammatory and analgesic experimental, so it provides a modern scientific basis for the thoery of toxicity reducing and efficacy enhancing. Acute toxicity test in mice proved that traditional Chinese medicine Tripterygium compatibility with licorice, the median lethal dose (LD50) has increased, although the difference between the groups was not significant, but for the compatibility of licorice triptolide attenuated role in providing an experimental basis.4. The research found that traditional Chinese medicine compatibility attenuated synergistic mechanism may be changed with the detoxification of drug toxicity drugs into existence after the blood and metabolic processes.