| Purpose:The study of utility and mechanism is the Sini powders and the different compatibilities in intervening experimental ulcerative colitis.Material and method:1 Study of Utility on the Sini Powders and the Different Compatibilities in Intervening Experimental Ulcerative ColitisThe experimental animals were divided into 14 groups and modeled with immunization. The states, colon organization and its pathological changes varying, thymus index, spleen index and activity of NF-?B of rats were treated as observation and measurement indicators. NF-?B mRNA was detected by Q-PCR technique. And it's protein activity was detected by immunohistochemistry.2 Study of Mechanism on the Sini Powders in Intervening Experimental Ulcerative Colitis Based on the first study, the experimental animals were divided into 5 groups and modeled with immunization. The states, colon organization and its pathological changes varying, IL-1?, TNF-?, IL-4, IL-10 of rats were treated as observation and measurement indicators. And we detected those cell factors mRNA with Q-PCR and activities with ELISA.Results:1 Study of Utility on the Sini Powders and the Different Compatibilities in Intervening Experimental Ulcerative Colitis1.1 The statesAnimalpatterns appeared diet reducedg, arch, polyethylene reactor and lazy action at the 14th day, and diet significantly reduced, diarrhea, hair dryed, arch, polyethylene reactor and lazy action at the 21st after modeling. Administrated orally, above symptoms the rats showed improvement with different degree.The increasing healthy rats' weight was 54%, but the modeling rats'weight increased averagely the range within 32%?38% before and after drug administration.1.2 Colon Organization and its Pathological ChangesWith visualinspection, we found the intestinal wall thicken, mucous membrane erosion, suiface of mucous membrane diffuse congestion and edema of the rates colons of model group. And blutpunkte and ulcer cound be observed. But all treatment groups were improved inordinately. The experimental result showed the model group was significant difference with normal group (P<0.05). Compared with model group, the degrees of colonic injury of all drug groups were significantly lower (P<0.05).Under light microscope, we observed there were ulceration, neutrophilicgranulocyte infiltration, congestion, erosion, epithelium deletion, lamina propria gland lessen, vessel hyperplasia of the rates colons of model group. The experimental result showed the model group was significant difference with normal group (P<0.05).Compared with model group, besides Chaishao group and Zhigan group, the rest groups were all significantly lower.1.3 Thymus Index and Spleen IndexThe different compatibilities can improve significantly thymus index and spleen index. After modeling the rates'thymus indexes of Sini powders group and Chaishao group were significantly lower than normal group, especially Chaishao group (P<0.05). Compared with model group, besides Chaishaozhi group ,Chaizhigan group, Chaizhi group and Shaozhi group, the rest groups were significant difference. the rates'spleen indexes of modeled rates (P<0.05). Compared with model group, the rates'spleen indexes of control group, Sini powders group and Chaizhigan group increased up significantly.1.4 NF-?BThe mRNA and activities of NF-?B of all drug groups decrease significantly, especially the Sini powders (P<0.05). The colon mucous membrane NF-?B mRNA of the rates of modle group were obviously higher than normal group's (P<0.05). Compared with model group, all drug groups were significant difference. In those, Zhigan group was significantly higher than model group (P<0.05).The colon mucous membrane NF-?B activity of the rates of modle group were obviously higher than normal group's (P<0.05). Sini powders group was no significant difference with noemal group. Compared with model group, besides Chaigan group and Zhigan group, the rest drug groups were significantly lower (P<0.05). Compared with Sini powders group, the rest drug groups increased significantly (P<0.05).2 Study of Mechanism on the Sini Powders in Intervening Experimental Ulcerative Colitis 2.1 IL-1?IL-1?mRNA levels of model group was higher than normal group (P<0.05). Compared with model group, all drug groups were significantly lower (P<0.05). IL-1?protein activity of model group were obviously higher than normal group (P<0.05). After feeding Chaishaozhi and Chaishao, IL-1?protein activity of UC rats obviously reduce (P<0.05). But after feeding Sini Powders, there rased significantly (P<0.05).2.2 TNF-?TNF-?mRNA and protein levels of model group are higher than normal group (P<0.05). After feeding experimental herbal medicine, TNF-?levels of UC rats obviously decrease (P<0.05), especially Sini Powders (P<0.05). Compared with Sini powders group, Chaishaozhi group increased significantly (P<0.05), but Chaishao group lowered (P<0.05).2.3 IL-4IL-4 mRNA levels of model group are lower than normal group (P<0.05). After feeding Sini Powders and Chaishao, IL-4 levels of UC rats obviously increased (P<0.05), especially Sini Powders (P<0.05). Compared with normal group, IL-4 protein activities of modeled rates were obviously decrease (P<0.05). After feeding experimental herbal medicine, Sini powders group and Chaishao group were obviously higher than model group (P<0.05). And Chaishao group was lowerer than Sini powders group (P<0.05).2.4 IL-10IL-10 mRNA levels of model group are lower than normal group (P<0.05). After feeding Sini Powders and Chaishao, IL-10 levels of UC rats obviously increased (P<0.05), especially Sini Powders (P<0.05). Compared with normal group, IL-10 protein activities of modeled rates were obviously decrease (P<0.05). After feeding experimental herbal medicine, Sini powders group and Chaishao group were obviously higher than model group (P<0.05). And Chaishao group was lowerer than Sini powders group (P<0.05).Conclusion:The Sini powders can intervene in experimental UC with stimulating the immune organs to excrete the immune cells and inhibit NF-?B activated.The Sini powders can intervene in experimental UC through adjusting cell factors levels. The mechanism of action chould include:?the Sini powders regulates the balance with inflammatory and anti-inflammatory cytokines through suppressing IL-1?move and TNF-?secret.and promoting IL-4 and IL-10 to secret;?the Sini powders can reduce the NF-?B levels of the experimental UC rats, and then regulat and control cytokine network. |
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